Core--Essential Laboratory Services

核心——必要的实验室服务

• 批准号: 6758079
• 负责人: Judith Anne Berliner
• 金额: $ 29.73万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6758079
• 关键词: atherosclerosis; biomedical facility; blood cells; blood chemistry; blood donor; blood lipoprotein; blood lipoprotein metabolism; clinical research; human subject; leukocytes; lipid metabolism; tissue /cell culture

The CHS portion of Core A provides human lipoproteins and leukocytes and general services to the Projects and other Cores. The MRL portion of Core A provides analytical chemistry assays for the Projects and other Cores. The CHS portion of Core A has provided essential laboratory services to our Program Project since its inception. All Projects and Cores have made extensive use of this Core. A major service provided by this Core is the preparation of blood products including human monocytes, lymphocytes and occassionally platelets as well as LDL, HDL and lipoprotein deficient serum (LPDS). Over the last year alone this Core isolated 10 billion monocytes, ten grams of lipoproteins, and 38 grams of LPDS. In addition, the Core provides standard assays, centralized glassware washing, autoclaving, maintenance of common facilities and radioisotope monitoring. This Core also isolates human aortic endothelial cells and smooth muscle cells and provides these as stock cultures to the Program's Investigators.

核心 A 的 CHS 部分为项目和其他核心提供人类脂蛋白和白细胞以及一般服务。核心 A 的 MRL 部分为项目和其他核心提供分析化学测定。核心 A 的 CHS 部分自项目启动以来一直为我们的项目提供必要的实验室服务。所有项目和核心都广泛使用了该核心。该核心提供的主要服务是制备血液制品，包括人单核细胞、淋巴细胞，偶尔还包括血小板以及低密度脂蛋白、高密度脂蛋白和脂蛋白缺乏血清 (LPDS)。仅去年一年，该 Core 就分离出了 100 亿个单核细胞、10 克脂蛋白和 38 克 LPDS。此外，核心还提供标准测定、集中玻璃器皿清洗、高压灭菌、公用设施维护和放射性同位素监测。该核心还分离了人主动脉内皮细胞和平滑肌细胞，并将它们作为储备培养物提供给该计划的研究人员。