Multi-frequency impedance sensor for cell analysis

用于细胞分析的多频阻抗传感器

• 批准号: 6933631
• 负责人: Jody V Vykoukal
• 金额: $ 9.79万
• 依托单位: ADEPTAS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933631
• 关键词: bioengineering /biomedical engineering; biomedical equipment; biomedical equipment development; blood cells; cell line; cell population study; dielectric property; electrical impedance; high throughput technology; human genetic material tag; human tissue; miniature biomedical equipment; portable biomedical equipment; tissue /cell culture

DESCRIPTION (provided by applicant): Broadband impedance analysis of individual cells yields a wealth of information about cell size, viability, morphology and interior structure. Cell dielectric properties including membrane conductivity, cytoplasmic conductivity, and membrane capacitance can be derived from multi-frequency impedance data and used to differentiate and enumerate cell populations in a mixed sample. Additionally, impedance analysis can be used to deduce cell differentiation, the onset of apoptosis, or cellular changes due to toxicant exposure. We have built a proof-of-principle particle analysis system that measures the impedance characteristics of individual cells at multiple frequencies as they pass through a sensor incorporated into a microfluidic flow channel. The goal of the effort described in this proposal is to improve our current particle impedance analyzer to yield a high-throughput, multipurpose unit for cell analysis. Specifically, a scalable impedance measurement circuit based on relatively low cost discrete components will be developed to allow for particle impedance spectroscopy at three frequencies. A more facile fabrication method for the impedance sensor channel will be developed to yield a credit-card sized, potentially disposable microfluidic device. The sensor channel cartridge and impedance sensing electronics will be integrated and the resulting prototype device used to characterize the dielectric properties of cultured cells and blood cell subpopulations. The long-term goal of this project is to realize an inexpensive, commercializable, hand-held multi-frequency particle impedance sensor that can be used for many cell analysis applications, including tissue culture, drug discovery, point-of-care diagnostics, and other scientific and industrial needs.

描述（由申请人提供）：单个细胞的宽带阻抗分析产生了有关细胞大小，活力，形态和内部结构的大量信息。细胞介电特性在内，包括膜电导率，细胞质电导率和膜电容性可以从多频阻抗数据中得出，并用于在混合样品中区分和枚举细胞种群。另外，阻抗分析可用于推断细胞分化，凋亡的发作或由于毒性暴露而引起的细胞变化。我们已经建立了一个原理粒子分析系统，该系统通过将传感器通过掺入微流体流动通道的传感器时，以多个频率的方式测量单个细胞的阻抗特性。本提案中描述的努力的目的是改善我们当前的颗粒阻抗分析仪，以产生高通量，多功能单元进行细胞分析。具体而言，将开发基于相对较低成本离散组件的可伸缩阻抗测量电路，以允许在三个频率下进行粒子阻抗光谱。为阻抗传感器通道的一种更容易的制造方法将开发出一种信用卡大小的，潜在的一次性微流体设备。将整合传感器通道盒和阻抗传感电子设备，并用于表征培养细胞和血细胞亚群的介电特性所产生的原型设备。该项目的长期目标是实现廉价，可商业化的手持式多频颗粒阻抗传感器，该传感器可用于许多细胞分析应用，包括组织培养，药物发现，护理点诊断和其他科学和工业需求。