Metal Enhancement for a Near-IR Fluorescence Scanner

近红外荧光扫描仪的金属增强

• 批准号: 6932939
• 负责人: Jon P Anderson
• 金额: $ 20.04万
• 依托单位: LI-COR BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-10 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932939
• 关键词: bioengineering /biomedical engineering; fluorescence; fluorescent dye /probe; infrared radiation; metals; molecular /cellular imaging; molecular film; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): Fluorescent molecules that are in close proximity to metallic surfaces, including silver and gold island films, produce increased fluorescence intensity along with increased photostability and decreased fluorescent lifetimes. These metal island films have been largely studied for their ability to increase fluorescent intensities in low quantum yield fluorophores, but little information exists on the ability of these metal islands to enhance the performance of fluorescent dyes used in proteomic and genomic applications. Moreover, the effects of metallic surface characteristics on the enhancement of fluorescent dyes have not been systematically studied, and even less is known about the reproducibility and longevity of these metal island films. The overall objective of this proposal is to enhance fluorescence of near-infrared dyes through the use of metal island films. Fluorescence in the near-infrared offers the additional advantage of significantly lowering the background generated by the visible wavelengths used in currently available array technologies. We will characterize techniques for producing metal island films with respect to reproducibility and longevity. We will also explore metal island geometry that may enhance fluorescence intensity. This work will add to our knowledge of how metallic surfaces can be reproducibly generated and will lay the foundation for a broader understanding of how metal enhanced fluorescence can be used to lower the limit of sample detection. In the long term, the goal of this research will be to develop a microarray slide or substrate that uses metal enhanced fluorescence to decrease the limit of detection while using currently available instrumentation.

描述（由申请人提供）：与金属表面非常接近的荧光分子（包括银和黄金岛膜）产生增加的荧光强度以及增加的光稳定性和荧光寿命降低。这些金属岛膜在低量子产量荧光团中提高荧光强度的能力已在很大程度上进行了研究，但是关于这些金属岛增强荧光染料在蛋白质组学和基因组应用中使用的荧光染料性能的能力的信息很少。此外，金属表面特征对增强荧光染料增强的影响尚未系统地研究，甚至对这些金属岛膜的可重复性和寿命的了解更少。 该提案的总体目的是通过使用金属岛膜来增强近红外染料的荧光。近红外的荧光提供了明显降低当前可用阵列技术中可见波长产生的背景的其他优势。我们将描述生产有关可重复性和寿命的金属岛膜的技术。我们还将探索可能增强荧光强度的金属岛几何形状。这项工作将增加我们对金属表面如何可重复产生的了解，并为更广泛地理解如何使用金属增强荧光来降低样品检测极限。从长远来看，这项研究的目的是开发使用金属增强荧光的微阵列幻灯片或底物，以减少使用当前可用仪器时的检测极限。