High Speed MultiChannel Timing System for Imaging

用于成像的高速多通道定时系统

• 批准号: 6961864
• 负责人: John Graham White
• 金额: $ 6.71万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6961864
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; confocal scanning microscopy; fluorescence microscopy; fluorescent dye /probe; image processing; molecular /cellular imaging; photonics; spectrometry

DESCRIPTION (provided by applicant): Laser scanning, multiphoton fluorescence microscopy has become the method of choice for visualizing developmental and cellular dynamics of living tissue. Recently two new imaging modalities have been developed that provide extra dimensions of data from fluorescence signals. Spectral imaging can allow multiple fluorescent probes to be resolved even if their spectra significantly overlap. Lifetime imaging can provide information about the molecular environment of a probe and also has considerable potential for making accurate FRET measurements, a technique that can measure molecular interactions in vivo. We are proposing to develop a very high-speed time-correlated photon counting system that has 32 parallel channels. ft is designed to be used with a spectrometer that has been developed for multiphoton microscopy so t h a t simultaneous spectral and lifetime data can be acquired at photon counting rates that are more than an order ot magnitude greater that what can be obtained with currently-available instrumentation. The system uses ultra high-speed timer integrated circuits that have recently become available. The proposed system should be economical to produce and is being designed so that it can be easily added to currently available laser scanning microscopes.

描述（由申请人提供）： 激光扫描，多光子荧光显微镜已成为可视化活组织发育和细胞动力学的首选方法。最近，已经开发出了两种新的成像方式，可提供来自荧光信号的数据的额外尺寸。光谱成像可以使多个荧光探针被解析，即使它们的光谱显着重叠。终生成像可以提供有关探针分子环境的信息，并且具有实现精确的FRET测量的相当大潜力，该技术可以在体内测量分子相互作用。我们建议开发一个非常高速的时间相关的光子计数系统，该系统具有32个并行通道。 FT设计用于用于多光子显微镜的光谱仪，因此可以以光子计数速率获得同时的光谱和寿命数据，该光子计数速率远远超过量幅度，即可以使用当前可用的仪器获得的量级。该系统使用最近可用的超高计时器集成电路。提出的系统应该是经济的，并且正在设计，以便可以轻松地将其添加到当前可用的激光扫描显微镜中。