Fluid Waveguides For Microfluidic Flow Cytometry

用于微流控流式细胞术的流体波导

• 批准号: 6993500
• 负责人: John S Oakey
• 金额: $ 25万
• 依托单位: METAFLUIDICS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2007-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6993500
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biotechnology; blood cell count; clinical biomedical equipment; clinical research; flow cytometry; fluorescent dye /probe; microarray technology; microfluidics; nanotechnology; platelets; portable biomedical equipment

DESCRIPTION (provided by applicant): This Phase I SBIR application proposes the use of novel fluid-fluid waveguiding techniques as the basis of an on-chip flow cytometry technology. We have shown that refractive index-mismatched fluids may be used to create dynamic waveguides and that these guides may be used to quantify the concentration of particulates contained within the core fluid. We propose to demonstrate the feasibility and advantages of this technology by performing integrated on-chip activated platelet counts. This proposed application serves as a poignant illustration of this technology's potential as a point of care biomedical diagnostic tool. The specific aims of this proposal are to: 1) To configure flow conditions and optics for single cell detection sensitivity. We believe that the sensitivity limit of this technique will enable the detection and counting of individual cells or particles. 2) To combine this novel on-chip waveguiding technique with Metafluidics' existing expertise in microscale fluid and cell handling to perform a single-step whole blood assay. Success of this aim will be quantified through validation against standardized activated platelet counting protocols. Phase I success will set the stage for the maturation of this technology during Phase II, which will result in a piece of permanent hardware with which customized cartridges for virtually any assay may be used. This will produce a unique instrument that is broadly applicable as a focused diagnostic tool or a platform research aid.

描述（由申请人提供）：此I阶段SBIR应用建议将新型流体流体波导技术用作片上流式细胞仪技术的基础。我们已经表明，折射率不匹配的流体可用于创建动态波导，并且这些指南可用于量化核心流体中包含的颗粒的浓度。我们建议通过执行集成的片上激活的血小板计数来证明该技术的可行性和优势。该提议的应用程序是该技术作为护理生物医学诊断工具的潜力的凄美说明。该提案的具体目的是： 1）配置流量条件和光学元件以进行单细胞检测灵敏度。我们认为，该技术的灵敏度极限将使单个细胞或颗粒的检测和计数。 2）将这种新颖的片上波动技术与元流体学的现有专业知识相结合 显微镜流体和细胞处理以执行单步全血分析。该目标的成功将通过验证标准化激活的血小板计数方案来量化。 第一阶段的成功将为这项技术在第二阶段的成熟奠定基础，这将导致一件永久性的硬件，几乎可以使用自定义的墨盒来使用任何测定法。这将产生一种独特的仪器，该工具广泛适用于专注的诊断工具或平台研究辅助工具。