Nanodots for Real-Time Monitoring of Signaling Pathways

用于实时监测信号通路的纳米点

• 批准号: 6994251
• 负责人: David Schultz
• 金额: $ 10万
• 依托单位: SEASHELL TECHNOLOGY, LLC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994251
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biological signal transduction; biomaterial interface interaction; biomedical equipment development; biosensor device; biotechnology; cell membrane; fluorescence microscopy; gold; intracellular transport; molecular /cellular imaging; molecular probes; monitoring device; nanotechnology; protein kinase C; silver

DESCRIPTION (provided by applicant): The ability to monitor signaling pathways in live cells in real time will provide a new experimental methodology for identifying and assessing modulators of signal transduction cascades. The objective of this proposal is to produce, functionalize, and verify the utility of a new type of protein and peptide conjugated nanoparticle reporter that will readily traverse the plasma membrane, concentrate in the cytoplasm and be responsive to signal transduction events. Signaling events will be detected in real-time by optically tracking the translocation of the fluorescent signal transduction domain conjugated nanoparticles to specific, signal dependent intracellular locations. In Phase II we will expand the assay to allow multiplex analysis of the regulation of cell signaling proteins in real-time will provide insight regarding the interplay of signaling cascades and provide a source for unique biosensors. These biosensors will be immediately relevant for assessing exposure to potential chemical or biological threat agents, identifying toxic substances, and discovering novel modifiers of cell signaling pathways. The tools developed in this Phase I proposal will aid medical researchers in their understanding of complex biological systems, thus providing opportunities for the development of new diagnostic or therapeutic compounds.

描述（由申请人提供）：实时监测活细胞中信号传导途径的能力将为识别和评估信号转导级联调节剂提供新的实验方法。该提案的目的是生产、功能化和验证新型蛋白质和肽缀合的纳米颗粒报告基因的效用，该报告基因将很容易穿过质膜，集中在细胞质中并对信号转导事件做出响应。通过光学跟踪荧光信号转导域缀合纳米颗粒到特定的信号依赖性细胞内位置的易位，可以实时检测信号事件。在第二阶段，我们将扩展检测方法，以允许对细胞信号蛋白的调节进行实时多重分析，这将提供有关信号级联相互作用的见解，并为独特的生物传感器提供来源。这些生物传感器将直接用于评估潜在化学或生物威胁剂的暴露、识别有毒物质以及发现细胞信号传导途径的新型修饰剂。 第一阶段提案中开发的工具将帮助医学研究人员了解复杂的生物系统，从而为开发新的诊断或治疗化合物提供机会。