Nanodots for Real-Time Monitoring of Signaling Pathways

用于实时监测信号通路的纳米点

• 批准号: 6994251
• 负责人: David Schultz
• 金额: $ 10万
• 依托单位: SEASHELL TECHNOLOGY, LLC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994251
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biological signal transduction; biomaterial interface interaction; biomedical equipment development; biosensor device; biotechnology; cell membrane; fluorescence microscopy; gold; intracellular transport; molecular /cellular imaging; molecular probes; monitoring device; nanotechnology; protein kinase C; silver

DESCRIPTION (provided by applicant): The ability to monitor signaling pathways in live cells in real time will provide a new experimental methodology for identifying and assessing modulators of signal transduction cascades. The objective of this proposal is to produce, functionalize, and verify the utility of a new type of protein and peptide conjugated nanoparticle reporter that will readily traverse the plasma membrane, concentrate in the cytoplasm and be responsive to signal transduction events. Signaling events will be detected in real-time by optically tracking the translocation of the fluorescent signal transduction domain conjugated nanoparticles to specific, signal dependent intracellular locations. In Phase II we will expand the assay to allow multiplex analysis of the regulation of cell signaling proteins in real-time will provide insight regarding the interplay of signaling cascades and provide a source for unique biosensors. These biosensors will be immediately relevant for assessing exposure to potential chemical or biological threat agents, identifying toxic substances, and discovering novel modifiers of cell signaling pathways. The tools developed in this Phase I proposal will aid medical researchers in their understanding of complex biological systems, thus providing opportunities for the development of new diagnostic or therapeutic compounds.

描述（由申请人提供）：实时监视活细胞中信号通路的能力将提供一种新的实验方法，用于识别和评估信号转导级联的调节剂。该提案的目的是生产，功能化和验证一种新型蛋白质和肽共轭纳米颗粒报告的实用性，该纳米粒子报告将容易穿越质膜，将其集中在细胞质中，并响应信号转导事件。信号事件将通过光学跟踪荧光信号转导域共轭纳米颗粒的易位，以实时检测到信号事件。在第二阶段，我们将扩展测定，以实时对细胞信号蛋白的调节进行多重分析，将提供有关信号级联相互作用的洞察力，并为独特的生物传感器提供源。这些生物传感器将立即与评估潜在的化学或生物威胁剂的接触，鉴定有毒物质以及发现细胞信号通路的新型修饰符。 在本阶段提案中开发的工具将帮助医学研究人员对复杂的生物系统的理解，从而为开发新的诊断或治疗化合物提供了机会。