Advanced, robotically-based, high-throughput radiation

先进的、基于机器人的高通量辐射

• 批准号: 7052503
• 负责人: DAVID JONATHAN BRENNER
• 金额: $ 97.35万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7052503
• 关键词: automated data processing; bioimaging /biomedical imaging; biomedical equipment development; biotechnology; bioterrorism /chemical warfare; clinical research; cooperative study; high throughput technology; human subject; image processing; lymphocyte; method development; micronucleus; radiation dosage; radiation therapy dosage; reticulocytes; robotics; tissue /cell culture

Our goal is to develop a fully automated ultra-high throughput radiation biodosimetry workstation, using purpose-built robotics and advanced high-speed automated image acquisition. Maximum throughput will be 30,000 samples / day, compared with throughputs in current devices of a few hundred samples / day. The basic system involves the well-characterized micronucleus assay in lymphocytes, with all the assays being carried out in-situ in multi-well plates. * By calling up pre-programmed options in timing, liquid handling, and image analysis, the device will also measure gamma-H2AX foci yields, and micronucleus yields in reticulocytes, both providing "same-day answer" dose estimates. * By calling up pre-programmed options in liquid handling steps, the device will also measure micronuclei in other readily-accessible tissues, such as exfoliated cells from urine or buccal smears. A key option of the system will be that each lymphocyte sample will be split in two, with one of the two split samples being irradiated to a dose of 1.8 Gy, before being analyzed. This will allow a positive control for each individual, providing an internal calibration to take into account inter-individual variability in radiosensitivity. We will develop both a Phase 1 and a Phase 2 device, with a 12-18 month lag between them: * The Phase 1 device, using 96-well plates, will have a throughput of 6,000 samples (3,000 individuals) per 15 hour day, and will use monochrome imaging. Peripheral blood drawn by venipuncture will be used. * The Phase 2 device, using 384-well plates, will have a throughput of 30,000 samples (15,000 individuals) per 15 hour day, and will use color imaging. Capillary blood from a finger stick or a high-throughput laser skin perforator will be used. Our Specific Aims, which will run in parallel throughout the project are 1) product development, and 2) the optimization / calibration / testing of biological protocols. Mass radiological triage will be critical after a large-scale event because of the need to identify, at an early stage, those individuals who will benefit from medical intervention, and those who will not. Our goal is to develop a fully automated ultra-high throughput biodosimetry workstation product (30,000 samples/day), using purpose-built robotics and advanced high-speed automated image acquisition.

我们的目标是使用专门构建的机器人技术和高级高速自动化图像采集开发全自动的超高吞吐量辐射生物测量工作站。与当前几百个样品中的吞吐量相比，每天的最大吞吐量为30,000个样品。基本系统涉及淋巴细胞中特征良好的微核测定法，其中所有测定在多孔板中进行原位。 *通过调用定时，液体处理和图像分析中的预编程选项，该设备还将测量网状细胞中的γ-H2AX焦点收益率和微核产量，均提供 “当天答案”剂量估计。 *通过调用液体处理步骤中的预编程选项，该设备还将测量其他易于接近的组织中的微核，例如尿液或颊涂片的去角质细胞。该系统的关键选择是每个淋巴细胞样品将分为两部分，在分析之前将两个分裂样品之一辐射为1.8 Gy的剂量。这将允许对每个人进行积极的控制，并提供内部校准，以考虑放射线敏感性的个体变异性。 我们将同时开发1阶段和2期设备，它们之间有12-18个月的滞后： *使用96孔板的1期设备每15小时的吞吐量为6,000个样品（3,000个个体），并将使用单色成像。将使用静脉穿刺吸收的外周血。 *使用384孔板的2期设备每15小时的吞吐量为30,000个样品（15,000个个体），并将使用彩色成像。将使用手指棒或高通量激光皮肤穿孔器的毛细血管血。我们的具体目标将在整个项目中并行运行是1）产品开发，以及2）生物方案的优化 /校准 /测试。大规模放射线分类在发生大规模事件后将至关重要，因为有必要在早期阶段确定那些将从医疗干预中受益的人，而那些不会从医疗干预中受益的人。我们的目标是开发全自动的超高吞吐量生物测量工作站产品（每天30,000个样品）， 使用专用的机器人技术和高级高速自动化图像采集。