BONE AND KNEE LOADING

骨骼和膝盖负载

基本信息

批准号：
6937255
负责人：
Hiroki Yokota
金额：
$ 7.58万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-01 至 2007-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6937255
关键词：
aging biological models biomechanics bone development computed axial tomography femur femur fracture histology knee laboratory mouse mechanical pressure mechanical stress morphometry skeletal stress

项目摘要

DESCRIPTION (provided by applicant): One of the major health needs in the elderly is to strengthen bone and prevent bone mass. In this project, we propose to investigate a novel mechanical loading model- knee joint loading - to stimulate bone formation and to strengthen mechanical properties of femur and a femoral neck. In our preliminary studies using mouse ulnae, bone formation was stimulated by loading an elbow joint. Furthermore, the loads required to induce bone formation with this elbow-loading model were smaller than that with a traditional longitudinal ulna-loading model. These results suggest that femur and a femoral neck can be strengthened by mechanical loads that are applied to a knee joint. The specific goal of the proposed project is to evaluate the effects of loads applied to the knee joint on bone mass and mechanical strength of femur and a femoral neck. The hypothesis, tested in the proposed study, is: "Low-amplitude sinusoidal mechanical loads with < 1 N at -20 Hz on a knee joint will stimulate new bone formation in femur and a femoral neck, and increase resistance to fracture." In the proposed study we will first identify the appropriate knee-loading conditions by examining streaming potentials and dissipation energy. Mechanical loads at physiological frequencies will be applied to the knee joint and streaming potentials and dissipation energy will be determined in the femur. Using the conditions that induce streaming potentials and elevate dissipation energy, we will then evaluate the loading effects through bone histomorphometry, micro-Computed Tomography (mu CT), and biomechanical tests. C57BL/6 mice will be given mechanical loads for 3 min per day for 3 consecutive days. After staining newly formed bone with calcein, bone morphometric parameters will be determined along a femoral midshaft. Three-dimensional morphology will be determined with mu CT, and mechanical parameters such as ultimate force, and work to failure will be determined. The proposed study will contribute to development of a novel loading model to strengthen bone without causing deformation of fragile bone in the elderly.

描述（由申请人提供）：老年人的主要健康需求之一是增强骨骼和预防骨骼。在这个项目中，我们建议研究一种新型的机械载荷模型 - 膝关节负荷 - 以刺激骨骼形成并增强股骨和股骨颈的机械性能。在我们使用小鼠尺骨的初步研究中，通过肘接头刺激骨骼形成。此外，使用该肘部负载模型诱导骨形成所需的负载小于传统的纵向尺骨载荷模型。这些结果表明，股骨和股骨颈可以通过施加在膝关节上的机械载荷来增强。拟议项目的具体目标是评估施加到膝关节对骨骼质量和股骨颈机械强度的载荷的影响。该假设在拟议的研究中进行了检验，是：“膝关节上-20 Hz <1 n的低振幅正弦机械载荷将刺激股骨和股骨颈部的新骨形成，并增加对骨折的耐药性。”在拟议的研究中，我们将首先通过检查流势和耗散能量来确定适当的膝关节加载条件。生理频率处的机械载荷将用于膝关节，并将在股骨中确定流势和耗散能量。利用诱导流势并提高耗散能量的条件，我们将通过骨骼组织形态计量学，微型计算层析成像（MU CT）和生物力学测试来评估负载效应。 C57BL/6小鼠将连续3天给予每天3分钟的机械载荷。用钙软件蛋白染色新形成的骨骼后，将沿股骨中层确定骨形态学参数。三维形态将用MU CT确定，并确定机械参数（例如最终力），并确定失败的工作。拟议的研究将有助于开发一种新型的负载模型，以增强骨骼，而不会引起老年人的脆弱骨骼的变形。