Automated Cell Preparation in Tubes for 3D Microscopy

用于 3D 显微镜的管内自动细胞制备

• 批准号: 6861456
• 负责人: Deirdre R. Meldrum
• 金额: $ 11.83万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-09 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6861456
• 关键词: bioimaging /biomedical imaging; biomedical automation; cell morphology; clinical research; cytodiagnosis; early diagnosis; flow cytometry; high throughput technology; human tissue; molecular probes; neoplasm /cancer diagnosis; technology /technique development; three dimensional imaging /topography; tissue /cell preparation

DESCRIPTION (provided by applicant): 3D microscopy represents a powerful new cell analysis tool for early detection and diagnosis of cancer, but its future use may be limited because methods for preparation of samples are cumbersome, inefficient, labor intensive and generally imprecise. Current methods for cytological sample collection are manual and distributed in nature through various physicians' office laboratories and local hospitals, with the actual analysis being centralized at regional clinical laboratories. Among the cytological specimens are sputum, gynecological and colorectal scrapes, fine needle aspirates, urinary tract, and gastrointestinal samples. We propose the development of a new automated system that will transform these difficult and messy clinical specimens into an optimal format for 3D microscopy morphological and molecular analysis. The model and method we propose is comprised of three sequential steps. First, at the distributed site, an automated sample processor dissociates and fixes cells and debris for shipment in an automation compatible canister. Second, after shipment to a centralized clinical laboratory, the specimen canisters are loaded into an automatic processor that performs cleanup (debris removal), specimen/assay specific staining (and counterstaining), and finally embedding of cells of interest in glass microcapillary tubes (about 50 mu m ID), with cells being spaced at regular intervals (about 200 mu m) within a tube. This preparation format is uniquely suited for integration with multiple 3D imaging platforms for true 3D volumetric assessment of cell morphology and molecular probe and/or stain density distribution. The proposed system also enables use of cytometric flow sorting for enrichment of cells of interest at an intermediate stage of the sample preparation process. The potential impact to improved human health through rapid diagnostic screening will be illustrated using a high impact emerging technology, optical tomography. In summary, the aim of the proposed project is to develop, design, and build a complete sample processing system that automates the process of sample cleanup, assay specific staining, and mounting of cells into glass microcapillary tubes, and a tube positioning and rotation scanner mechanism for 3D microscopy analysis of cell morphology for the early detection of cancer.

描述（由申请人提供）：3D显微镜代表了一种用于癌症的早期检测和诊断的强大新细胞分析工具，但是它的未来使用可能是有限的，因为制备样品的方法繁琐，效率低下，劳动量很大，并且通常不精确。当前的细胞学样本收集方法是手动的，并通过各种医师的办公实验室和当地医院在本质上分发，实际分析集中在区域临床实验室中。在细胞学标本中，有痰液，妇科和结直肠擦伤，细针抽吸物，尿路和胃肠道样品。我们提出了一种新的自动化系统的开发，该系统将这些困难而混乱的临床标本转变为3D显微镜形态和分子分析的最佳格式。我们建议的模型和方法由三个顺序步骤组成。首先，在分布式站点上，自动样品处理器解离并修复了在自动化兼容罐中发货的单元和碎屑。其次，在运往集中式临床实验室后，将样品罐加载到自动处理器中，该处理器进行清理（清除碎屑），样品/测定特定的染色（和对抗染色），最后嵌入了玻璃微毛皮管中感兴趣的细胞中的细胞（约50 MU M ID）（约50 MU M ID）（约50 MU M ID），并在常规的间隔内（约200 MU MU）（约200 MU MU）。这种制备格式独特地适合与多个3D成像平台集成，用于对细胞形态和分子探针和/或染色密度分布的真实3D体积评估。所提出的系统还可以利用细胞流量分类在样本制备过程的中间阶段富集感兴趣的细胞。通过快速诊断筛查对改善人类健康的潜在影响将使用高影响的新兴技术（光学断层扫描）进行说明。总而言之，拟议项目的目的是开发，设计和建立一个完整的样品处理系统，该系统可以自动化样品清理，测定特定的染色以及将细胞安装到玻璃微毛细管中，以及对早期检测癌症的细胞形态学分析的管定位和旋转扫描仪机械的定位和旋转扫描仪机械。