Regulation of TA Division in Drosophila Spermatogenesis

TA分裂在果蝇精子发生中的调控

• 批准号: 6991794
• 负责人: ALEXIS NAGENGAST
• 金额: $ 3.09万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6991794
• 关键词: Drosophilidae; biological signal transduction; cell differentiation; cell growth regulation; cell proliferation; molecular cloning; postdoctoral investigator; spermatogenesis; stem cells

DESCRIPTION (provided by applicant): Stem cells comprise a unique population of cells that divide asymmetrically to balance self-renewal with differentiation. The daughter of a stem cell undergoes transit-amplification, where a tightly controlled number of mitotic divisions expand the cell pool before terminal differentiation. However, the mechanisms regulating this process remain poorly understood. As in mammals, Drosophila spermatogenesis also utilizes transit-amplification to produce spermatocytes from germline stem cells. Recent work in the DiNardo lab suggests that the BMP pathway is necessary for transit amplifying (TA) spermatogonial cells to transition into spermatocyte differentiation. Additionally, counting mutants, which increase (count up; ctu) or decrease (count down; cdn) the number of TA divisions by one has been identified. Cross species transplantation experiments will test the model that a counting mechanism intrinsic to TA germ cells monitors division number. Cloning cdn and positioning ctu and cdn in the circuit regulating the TA gonia to spermatocyte transition will further examine if somatic cells signal to the germ cells to trigger the shift to spermatocyte differentiation. Such feedback may provide for the coordinated differentiation of somatic and germline cells.

描述（由申请人提供）：干细胞包含一个独特的细胞群，它们不对称地分裂以平衡自我更新与分化。干细胞的女儿经历了转运扩大，其中有丝分裂的数量紧密控制在末端分化之前扩大了细胞池。但是，调节这一过程的机制仍然很少理解。与哺乳动物一样，果蝇的精子发生也利用了转运扩大来产生生殖器干细胞的精子细胞。 Dinardo Lab中的最新工作表明，BMP途径对于过渡（TA）精子细胞以过渡到精子细胞分化是必需的。此外，计数突变体的增加（计数； ctu）或减少（倒数; cdn），已经确定了TA分裂的数量。跨物种移植实验将测试TA生殖细胞固有的计数机制监测的模型。将CDN的克隆和定位CTU和CDN定位在调节ta gonia到精子细胞过渡的电路中，将进一步检查体细胞是否向生殖细胞发出信号，以触发向精子细胞分化的转移。这种反馈可以提供体细胞和种系细胞的协调分化。