Investigation of a development checkpoint in flies

果蝇发育检查点的调查

• 批准号: 6884186
• 负责人: ADRIAN J HALME
• 金额: $ 4.4万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6884186
• 关键词: Drosophilidae; biological signal transduction; cell cell interaction; cell differentiation; cell growth regulation; cell proliferation; cytogenetics; endocrine gland /system; gene expression; genetic screening; hormone regulation /control mechanism; metamorphosis; neoplastic growth; postdoctoral investigator; tissue /cell culture

DESCRIPTION (provided by applicant): In order for a multicellular organism to develop properly, it must successfully coordinate the growth and differentiation of tissues across the entire organism. In cancer, the regulatory interactions between cell division and organismal development break down. In some instances, proliferating cancer cells can commandeer endogenous endocrine signals to promote tumor growth and malignancy. The experiments in this proposal investigate the perturbation of endocrine signaling by both regenerative and tumorous cell division at the initiation of metamorphosis in holometabolous insects. In the fruit fly, Drosophila melanogaster, entry into metamorphosis is promoted by the ecdysteroid hormone 20-hydroxyecdysone. However, this endocrine activation of metamorphosis is delayed if larval tissues continue to undergo additional rounds of cell division. By utilizing: a) the ability to regulate hormone levels in Drosophila by feeding exogenous hormone to larvae and adult flies, b) cell and tissue culture and c) genetic screens, I plan to identify the important genes and molecules that mediate the interaction between dividing cells and the Drosophila endocrine system.

描述（由申请人提供）： 为了使多细胞生物正确发育，它必须成功地协调整个生物体组织的生长和分化。在癌症中，细胞分裂与生物发育之间的调节相互作用分解。在某些情况下，增殖的癌细胞可以指挥内源性内分泌信号以促进肿瘤的生长和恶性肿瘤。该提案中的实验研究了自代谢昆虫的变态启动时再生和肿瘤细胞分裂的内分泌信号传导的扰动。在果蝇中，果蝇果蝇（Drosophila Melanogaster），ecdysteroid激素20-羟基甲虫促进了变形。但是，如果幼虫组织继续进行其他细胞分裂，则这种变形的内分泌激活会延迟。通过利用：a）通过向幼虫和成年蝇喂养外源激素来调节果蝇中激素水平的能力，b）细胞和组织培养以及c）遗传筛选，我计划识别介导分裂细胞与果蝇内分泌系统之间相互作用的重要基因和分子。