In Vitro High Throughput Screening Assay for MKP-3(RMI)

MKP-3(RMI) 体外高通量筛选试验

• 批准号: 7058166
• 负责人: JOHN S. LAZO
• 金额: $ 0.45万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2006-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7058166
• 关键词: biological signal transduction; biotechnology; combinatorial chemistry; drug screening /evaluation; high throughput technology; mitogen activated protein kinase; phosphatase inhibitor; protein tyrosine phosphatase; small molecule; technology /technique development

DESCRIPTION (provided by applicant): Mitogen activated protein kinase phosphatase-3 (MKP-3) is a dual specificity phosphatase that has a central role regulating intracellular signal transduction by controlling the phosphorylation status of mitogen activated protein kinases. There is considerable evidence indicating MKP-3 could be a potential molecular target for several human diseases. Nonetheless, no potent or selective small molecule inhibitors of this protein phosphatase have been identified. This proposal will provide sufficient reagents to permit a high throughput screening of 100,000 compounds

描述（由申请人提供）：丝裂原激活蛋白激酶磷酸酶-3（MKP-3）是一种双特异性磷酸酶，通过控制丝裂原激活蛋白激酶的磷酸化状态来调节细胞内信号转导。有大量证据表明 MKP-3 可能是多种人类疾病的潜在分子靶标。尽管如此，尚未发现这种蛋白磷酸酶的有效或选择性小分子抑制剂。该提案将提供足够的试剂来高通量筛选 100,000 种化合物