3D STRUCT OF ENZYMES INTERACTING W/ NUCLEOTIDES: ESTROGEN BINDING

酶与核苷酸相互作用的 3D 结构：雌激素结合

• 批准号: 6667809
• 负责人: SHENG-XIANG LIN
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667809
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

Data were collected at MIT from crystals of CHK SH2 and a data set complete to 3Angstroems was obtained. Molecular replacement was attempted but a solution was not found. The most logical path to take now would have been to search for heavy atom derivatives and collect data. Unfortunately at the time our Laboratory was not equipped with an X-ray facility and we were unable to secure more time at an external establishment. We therefore applied for time at F2 in order to get MAD phases or as a contingency to collect heavy atom data. In the limited time we had at CHESS (around 2.5 days or less) we were unable to find suitable cryo-conditions for the crystals and had to resort to collecting data from potential heavy-atom derivatives. Four potential heavy atom data sets were collected at CHESS F2 and we are in the process of using this data to obtain starting phases for the SH2 domain. Small crystals of the SH2 domain complexed with the receptor peptide ERB-B2 which is expressed in mammalian breast cancer cells have also been grown recently and efforts are being directed to increase the size of these crystals. The crystal structure of the complex would be of fundamental importance in understanding the role of CHK in signalling in breast cancer.

数据是在麻省理工学院从 CHK SH2 晶体和数据集收集的 获得了完整的3埃。 分子替代是 尝试过但没有找到解决方案。 最符合逻辑的路径 现在是寻找重原子衍生物并收集 数据。 不幸的是，当时我们的实验室没有配备 X 射线设备，我们无法确保更多时间 外部设立。 因此我们申请了F2的时间，以便 获得 MAD 阶段或作为应急收集重原子数据。 在 我们在 CHESS 的时间有限（大约 2.5 天或更短） 无法找到适合晶体的冷冻条件，不得不 采取从潜在的重原子衍生物收集数据的方法。 四 潜在的重原子数据集是在 CHESS F2 收集的，我们 在使用这些数据来获取起始阶段的过程中 SH2 域。 SH2结构域的小晶体与 受体肽 ERB-B2 在哺乳动物乳腺癌中表达 最近也培养了细胞，并且正在努力 增加这些晶体的尺寸。 的晶体结构 复杂对于理解角色至关重要 CHK 在乳腺癌信号传导中的作用。