CREATION OF MOUSE GENE DISRUPTION FOR LYSOSOMAL MANNOSIDASE

溶酶体甘露糖苷酶小鼠基因破坏的产生

• 批准号: 6653552
• 负责人: KELLEY W. MOREMEN
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653552
• 关键词: Mammalia; bioimaging /biomedical imaging; biological products; biomedical resource; enzymes; microscopy; structural biology; technology /technique

The 3-dimensional structural of the Golgi processing enzyme, mannosidase II, is being determined by reconstruction from high resolution images from darkfield electron microscopy of the purified protein. Structural resolution at the 10E level has been obtained for this 116 kDa protein and optimization to higher resolution by further advances in image analysis will be undertaken in the coming year.

高尔基体加工酶的三维结构， 甘露糖苷酶 II，正在通过高重建来确定 纯化后的暗场电子显微镜分辨率图像 蛋白质。 已获得 10E 级别的结构分辨率 该 116 kDa 蛋白质并通过进一步优化至更高分辨率 图像分析方面的进展将在来年取得进展。