CORE--CELL PROCUREMENT AND PROCESSING RESOURCE

核心--电池采购及加工资源

• 批准号: 6668341
• 负责人: Shelly Heimfeld
• 金额: $ 25.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6668341
• 关键词: animal tissue; biomedical facility; cell population study; cooperative study; flow cytometry; hematopoietic stem cells; human subject; human tissue; tissue /cell culture

Cell procurement and processing resource. The Cell Procurement and processing Core will supply resources to help circumvent a critical limitation that impedes progress in the field of ex-vivo gene therapy, an inability to obtain sufficient qualities of purified stem cells or other cell populations needed for experimentation and optimization of the conditions required for efficient gene transduction. This Core will address that limitation by providing two specific functions. (1) The Core will make available to PEGT researchers as well as other NHLBI investigators large quantities of enriched stem cells or other specific blood cell populations for their in vitro studies, for primate model testing, and for pre-clinical trial usage. The Processing Core will obtain specimens form the Primate Core, and will operate a donor recruitment program, a cadaveric bone marrow program, and a Repository of Cryopreserved Research Specimens to supply the cell populations needed for large-scale cell selections. (2) Taking advantage of the instrumentation and expertise available from within the FHCRC Cryobiology Laboratory, the Core will provide clinical-grade cell processing and selections from both the human and primate components. The Core will also characterize these enriched and/or transduced cell populations by performing 2 and 3 color flow cytometric determinations, and quantitative CFC and LTC-IC assays. These diagnostic services will provide investigators with the ability to quality control results from the cell selections, and to determine efficiencies of transduction in the various stem/progenitor cell compartments. The ability of this Core to obtain, process, isolate and characterize large numbers of purified cells, and to deliver those cells to NHLBI researchers, will significantly enhanced studies in the areas of basic stem cell biology, and greatly facilitate the clinical advancement of novel gene therapy-based applications.

细胞采购和加工资源。细胞采购和处理核心将提供资源，以帮助规避阻碍离体基因治疗领域进展的关键限制，即无法获得实验和优化条件所需的足够质量的纯化干细胞或其他细胞群有效的基因转导所必需的。该核心将通过提供两个特定功能来解决该限制。 (1) Core 将为 PEGT 研究人员以及其他 NHLBI 研究人员提供大量富集干细胞或其他特定血细胞群，用于体外研究、灵长类动物模型测试和临床前试验使用。处理核心将从灵长类动物核心获取样本，并将运营捐赠者招募计划、尸体骨髓计划和冷冻保存研究样本库，以提供大规模细胞选择所需的细胞群。 (2) 利用 FHCRC 冷冻生物学实验室内提供的仪器和专业知识，该核心将提供人类和灵长类成分的临床级细胞处理和选择。 Core 还将通过执行 2 色和 3 色流式细胞术测定以及定量 CFC 和 LTC-IC 测定来表征这些富集和/或转导的细胞群。这些诊断服务将为研究人员提供对细胞选择结果进行质量控制的能力，并确定各种干细胞/祖细胞区室中的转导效率。该核心能够获取、处理、分离和表征大量纯化细胞，并将这些细胞交付给NHLBI研究人员，将显着加强基础干细胞生物学领域的研究，并极大地促进新基因的临床进展基于治疗的应用。