Identification of type III secreted Chlamydia proteins

III 型分泌型衣原体蛋白的鉴定

• 批准号: 6626251
• 负责人: THERESA D HO
• 金额: $ 4.64万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6626251
• 关键词: Chlamydia trachomatis; Escherichia coli; MHC class I antigen; Salmonella typhimurium; adenylate cyclase; antigen presentation; bacteria infection mechanism; bacterial proteins; bactericidal immunity; binding sites; cell line; chlamydial disease; cyclic AMP; cytotoxic T lymphocyte; flow cytometry; fusion gene; gene expression; genetic library; polymerase chain reaction; protein localization; protein signal sequence; protein structure function; protein transport; recombinant proteins; reporter genes; secretory protein; vaccinia virus

DESCRIPTION (provided by applicant): A growing body of evidence suggests that Chlamydiae encode a type III secretion system to translocate bacterial proteins into the host cell. Many studies of type III secreted proteins from other organisms use a truncated adenylate cyclase protein (CyaA) with no endogenous translocation domain to demonstrate that a protein under study is a substrate for a type III secretion system. This study will use a similar approach to identify Chiamydia trachomatis proteins that are translocated into the host cell cytoplasm via a type III secretion system. These type III secreted C. trachomatis proteins will be further characterized by determining where the proteins localize during C. trachomatis infection and how these proteins are regulated. Because the type III secreted Chiamydia proteins may be translocated into the host cell cytoplasm, the study will also demonstrate 1) if the C. trachomatis proteins are processed and presented by MHC-I in C. trachomatis-infected cells in vitro and 2) whether the CD8+ T cell responses to secreted C. trachomatis proteins can provide protection against a C. trachomatis challenge in vivo. Determination and characterization of translocated C. trachomatis proteins may help to identify targets for immune interventions or theraputic treatment of C. trachomatis infection.

描述（由申请人提供）：越来越多的证据表明 衣原体编码 III 型分泌系统以易位细菌蛋白 进入宿主细胞。许多关于 III 型分泌蛋白的研究 生物体使用截短的腺苷酸环化酶蛋白（CyaA），没有内源性 易位结构域证明所研究的蛋白质是底物 对于 III 型分泌系统。本研究将使用类似的方法 识别易位至宿主的沙眼衣原体蛋白 通过 III 型分泌系统进入细胞质。这些 III 型分泌 C. 通过确定沙眼衣原体蛋白的位置，可以进一步表征沙眼衣原体蛋白的特征。 沙眼衣原体感染期间蛋白质的定位以及这些蛋白质是如何定位的 受监管。因为 III 型分泌的衣原体蛋白可能会易位 进入宿主细胞的细胞质，该研究还将证明 1) 如果 C. 沙眼衣原体蛋白由衣原体中的 MHC-I 加工和呈递。 体外沙眼感染细胞和 2) CD8+ T 细胞是否对 分泌的沙眼衣原体蛋白可以提供针对衣原体的保护。 体内沙眼衣原体攻击。测定和表征 易位的沙眼衣原体蛋白可能有助于识别免疫靶点 沙眼衣原体感染的干预或治疗。