CORE--MYOCARDIAL CELL BIOLOGY AND VIRAL VECTOR FACILITY

核心——心肌细胞生物学和病毒载体设施

• 批准号: 7098691
• 负责人: Kirk U Knowlton
• 金额: $ 15.69万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7098691
• 关键词: Adenoviridae; adeno associated virus group; biomedical facility; cardiac myocytes; cell biology; laboratory mouse; laboratory rat; myocardium; tissue /cell culture; transfection; transfection /expression vector

DESCRIPTION (provided by applicant): Each of the projects in the program will utilize the Cell Biology Core Unit, which is designed to: 1) to provide purified cultures of neonatal rat ventricular myocardial cells and related techniques for gene transfer in these cells; 2) to provide purified mouse adult and neonatal ventricular muscle cells from various transgenic lines for analysis; 3) to generate and utilize recombinant adenoviral vectors for gene transfer in neonatal rat, adult and neonatal mouse myocardial cells, and for gene transfer into intact muscle for various projects in the program; and 4) to generate recombinant adeno-associated viral vectors for gene transfer in intact muscle.

描述（由申请人提供）： 该程序中的每个项目都将利用细胞生物学核心单元，该单元旨在： 1）提供新生大鼠心室心肌细胞及相关的纯化培养物 这些细胞中基因转移的技术； 2）提供纯化的小鼠成人和新生儿 来自各种转基因线的心室肌肉细胞用于分析； 3）生成和使用 重组腺病毒载体，用于新生大鼠，成年和新生小鼠的基因转移 心肌细胞，以及用于该程序中各个项目的基因转移到完整的肌肉中； 4）生成完整基因转移的重组腺相关病毒载体 肌肉。