Core B--Gene Transfer

核心B——基因转移

• 批准号: 6853159
• 负责人: Beverly L. Davidson
• 金额: $ 12.41万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6853159
• 关键词: Adenoviridae; Lentivirus; Retroviridae; adeno associated virus group; biomedical facility; biotechnology; cystic fibrosis; gene therapy; information systems; plasmids; recombinant virus; technology /technique development; transfection; transfection /expression vector

The Gene Transfer Core at the University of Iowa is integrated into multiple gene therapy projects directed at the study of diseases of the lung with particular application to cystic fibrosis. Gene Transfer Core staff are active participants in the development of gene transfer technologies. Interactions between Gene Transfer Core staff and colleagues, Zabner, Welsh, and McCray allows for cross-fertilization of ideas, technical advancements, and innovations in vector design. Additionally the Gene Transfer Core benefits from interactions with investigators in the Cardiovascular, Macular Degeneration and CF Centers. The Gene Transfer facility's overall objective is to support Drs. Zabner, Welsh and McCray in the use of gene transfer technologies. This includes consultation with them, development of novel vectors, collaborative testing of vectors generated for function and purity, and finally routine preparation. The Gene Transfer Core staff and investigators are in close contact through all phases of vector design and generation. Thus, the Core serves as both a research and development facility for gene transfer studies, and a service facility for routine vector preparations. As a part of the service the Gene Transfer Core will provide purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), and retrovirus (including lentivirus). This facility will also provide access to standard cell lines, expression plasmids, and stocks of recombinant reporter viruses. The main responsibilities of the Core will be: Prepare recombinant vectors; Quality control; Vector Dissemination; Maintain a database of vector stocks available for use; Catalogue plasmid database of expression vectors; develop new expression vectors as needed; Develop novel methods for virus production; Design and develop novel vectors.

爱荷华大学的基因转移核心被整合到针对肺部疾病研究的多个基因治疗项目中，并特别适用于囊性纤维化。基因转移核心人员是积极参与基因转移技术的参与者。基因转移核心员工与同事，Zabner，Welsh和McCray之间的互动允许交叉侵入思想，技术进步和向量设计中的创新。另外，基因转移核心受益于与心血管，黄斑变性和CF中心中研究人员的相互作用。基因转移设施的整体 目的是支持Drs。 Zabner，Welsh和McCray在使用基因转移技术方面。这包括与他们的咨询，新颖矢量的开发，为功能和纯度生成的向量的协作测试，以及最终的常规准备。基因转移核心人员和调查人员通过向量设计和发电的所有阶段密切接触。因此，核心既是基因转移研究的研究和开发设施，也是常规向量准备的服务机构。作为服务的一部分，基因转移核心将提供重组腺病毒，腺相关病毒（AAV）和逆转录病毒（包括慢病毒）的纯化和浓缩制剂。该设施还将提供重组记者病毒的标准细胞系，表达质粒和库存的访问。 核心的主要职责将是：准备重组向量；质量控制； 向量传播；维护可供使用的矢量库存数据库；表达向量的目录质粒数据库；根据需要开发新的表达向量；开发用于病毒生产的新方法； 设计和开发新颖的向量。