THE XIPHOPHORUS GENETIC STOCK CENTER

剑尾鱼遗传库存中心

• 批准号: 6890926
• 负责人: RONALD B WALTER
• 金额: $ 35.35万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6890926
• 关键词: Internet; animal colony; artificial chromosomes; computer program /software; computer simulation; computer system design /evaluation; cryopreservation; fish; freezing; genetic library; genetic mapping; genetic markers; genetic polymorphism; genetic registry /resource /referral center; genetic strain; genome; genotype; information dissemination; information systems; method development; molecular cloning; polymerase chain reaction; quantitative trait loci; sperm

This is a P40 resource proposal to maintain and expand the Xiphophorus Genetic Stock Center (XGSC), and enhance services provided to research investigators. Xiphophorus is a small freshwater, aquarium fish that has been extensively used in carcinogenesis studies, but also used as a model organism in a variety of different fields of study that transcends several NIH institutes. The XGSC has operated within the United States for over 70 years. The proposed initiatives will greatly enhance the usability of the Xiphophorus model system for research scientists using this system, but also for "non-Xiphophorus" scientists that envision application of the powerful genetics provided by this model system. The traditional strength of this model involves the non-biased assessment of genetic variability among Xiphophorus species (22 known taxa), and capability of producing fertile interspecies hybrids, allows chromosomal/marker inheritance to be followed in individual backcross hybrid progeny. Complex traits, such as susceptibility to the development of neoplastic disease, or behavioral characteristics, can be studied using Xiphophorus, and underlying genetic factors can be identified. Since research using these fishes is currently expanding within the scientific community, it is timely to enhance the services offered by the Xiphophorus Genetic Stock Center. We propose: 1) To preserve and enhance the Xiphophorus Genetic Stock Center and provide the research community with animals from genetically managed lines in large numbers according to the specifications of each investigator. 2) To establish and maintain extensive and flexible computer databases of fish, and derived samples. These databases will be made available for scientific use. Links and information regarding fish maintenance, current research projects and other XGSC information will be provided via the xiphophorus.org web- site. 3) To continue saturation of the Xiphophorus gene map and development of technologies providing investigators with efficient molecular tools necessary to use this powerful genetic system to approach complex scientific questions, and 4) To establish technology allowing cryopreservation of Xiphophorus sperm samples and thereby provide a preservation program where the XGSC maintains the genetic heritage of all Xiphophorus strains and species.

这是一项P40资源建议，旨在维护和扩展Xiphophorus遗传储备中心（XGSC），并增强为研究研究人员提供的服务。 Xiphophorus是一种小型淡水水族馆鱼类，已在癌变研究中广泛使用，但在各种不同的研究领域中也被用作模型生物，超越了几个NIH机构。 XGSC在美国在美国运营已有70多年的历史。 提出的计划将大大提高使用该系统的研究科学家的Xiphophorus模型系统的可用性，也将为“非Xiphophorus”科学家提高，这些科学家设想了该模型系统提供的强大遗传学的应用。 该模型的传统强度涉及对Xiphophorus物种（22个已知分类单元）之间的遗传变异性的无偏见评估，以及产生肥沃的种间杂种的能力，允许在单个后跨杂交后代中遵循染色体/标记遗传。 可以使用Xiphophorus研究复杂的特征，例如对肿瘤疾病发展或行为特征的敏感性，并可以确定潜在的遗传因素。 由于使用这些鱼类的研究目前正在科学界内扩展，因此及时加强Xiphophorus遗传储备中心提供的服务。我们建议：1）根据每个研究者的规格，保留和增强Xiphophorus遗传储备中心，并为研究界提供大量基因管理线的动物。 2）建立和维护鱼类的广泛而灵活的计算机数据库，并得出样品。这些数据库将用于科学用途。 有关鱼类维护，当前研究项目和其他XGSC信息的链接和信息将通过xiphophorus.org网站提供。 3）继续饱和Xiphophorus基因图和技术的开发，为研究人员提供了使用这种强大的遗传系统来解决复杂科学问题所需的有效分子工具，4）建立技术，允许对Xiphophorus精子样品进行冷冻保存，从而提供保存XGSC维持所有Xiphophorus菌株和物种的遗传遗产的程序。