RNA Polymerase Sigma Factors in Streptococcus pyogenes

化脓性链球菌中的 RNA 聚合酶 Sigma 因子

• 批准号: 6621993
• 负责人: Charles P. Moran
• 金额: $ 26.6万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-15 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6621993
• 关键词: DNA directed RNA polymerase; Streptococcus pyogenes; bacterial genetics; biological signal transduction; disease /disorder model; gene expression; gene mutation; genetic promoter element; genetic regulation; host organism interaction; laboratory mouse; nuclear runoff assay; site directed mutagenesis; skin infection; transcription factor; virulence

Streptococcus pyogenes (the group A streptococcus or GAS) is an important and common human pathogen. The diseases it causes range from self-limiting skin and throat infections, with, however, the potential for serious sequelae including rheumatic heart disease, acute glomerulonephritis, and possibly pediatric neuropsychological disorders, to severe invasive diseases like myositis and streptococcal toxic shock syndrome. Since single strains of the GAS seem to be able to cause most or all of these diseases, regulation of the expression of GAS genes in response to specific environmental differences within the host is probably key in determining the course of the infectious process. We propose here a new approach to learn more about control of gene expression in the GAS by the characterization of a new RNA polymerase (RNAP) secondary sigma factor needed to transcribe genes under different conditions and the characterization of the genes it regulates.

化脓性链球菌（A 族链球菌或 GAS）是一种重要且常见的人类病原体。 它引起的疾病范围广泛，从自限性皮肤和喉咙感染，到严重的侵袭性疾病，如肌炎和链球菌中毒性休克综合征，都有可能导致严重的后遗症，包括风湿性心脏病、急性肾小球肾炎和可能的小儿神经心理疾病。 由于 GAS 的单一菌株似乎能够引起大多数或全部这些疾病，因此根据宿主内特定环境差异调节 GAS 基因的表达可能是确定感染过程过程的关键。 我们在这里提出了一种新方法，通过在不同条件下转录基因所需的新RNA聚合酶（RNAP）次级西格玛因子的表征以及它所调节的基因的表征来了解更多关于GAS中基因表达的控制。