Chondrogenesis in Enzymatically Degradable Hydrogels

酶降解水凝胶中的软骨形成

• 批准号: 7120399
• 负责人: Jason A Burdick
• 金额: $ 9.72万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-05 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7120399
• 关键词: athymic mouse; biodegradable product; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterials; bone marrow; cartilage development; cartilage disorder; chondrocytes; copolymer; gel; nonhuman therapy evaluation; photochemistry; polymerization; polymethacrylate; protein degradation; stem cells; swine; tissue /cell culture; tissue engineering; transforming growth factors

DESCRIPTION (provided by applicant): The overall purpose of this K22 Scholar Development and Faculty Transition Career Award is to provide additional (1 year) postdoctoral mentorship and support to the principle investigator in the area of advancing photopolymerizable hydrogels for cartilage tissue engineering in addition to support (4 years) as an independent faculty member to continue research in this field. The proposed research originated to complement the principle investigator's background in the development of novel photocrosslinkable biomaterials for bone tissue engineering with a long-term objective of developing complex materials at the interface of bone and cartilage for craniofacial applications. It is the general goal of the principle investigator to develop an internationally recognized research laboratory that uses the synthesis and modification of novel materials as a platform for the development of clinically relevant therapeutics. The proposed research and career development program outlined in this proposal were developed by the principle investigator and Mentors from both the Massachusetts General Hospital and Massachusetts Institute of Technology in an effort to achieve this goal. Photopolymerizable hydrogels have many properties that are beneficial for cartilage tissue engineering including: the ability to form in vivo to fill complex defects with potential adhesive and integrative properties, both spatial and temporal control over the polymerization process, and biological signals are readily incorporated into the networks to control both cellular differentiation and tissue formation. These properties would be extremely beneficial for the regeneration of cartilage in craniofacial reconstruction. With these benefits in mind, the primary aims of the proposed research are to: . synthesize and characterize photopolymerizable copolymer hydrogel networks that resorb via an enzymatic degradation process, . investigate the quantity, distribution, and type of tissue formed by chondrocytes encapsulated in hydrogel networks as a function of hydrogel composition, . incorporate signals into hydrogel networks to control the chondrogenic differentiation of photoencapsulated mesenchymal stem cells, and . analyze critical issues of the photopolymerized constructs necessary for clinical application of these techniques.

描述（由申请人提供）：该 K22 学者发展和教师过渡职业奖的总体目的是为软骨组织工程光聚合水凝胶领域的主要研究者提供额外（1 年）的博士后指导和支持，此外支持（4年）作为独立教员继续在该领域进行研究。拟议的研究最初是为了补充主要研究者开发用于骨组织工程的新型光交联生物材料的背景，其长期目标是开发用于颅面应用的骨和软骨界面的复杂材料。主要研究者的总体目标是建立一个国际认可的研究实验室，利用新材料的合成和修饰作为开发临床相关疗法的平台。本提案中概述的拟议研究和职业发展计划是由麻省总医院和麻省理工学院的首席研究员和导师制定的，旨在实现这一目标。 可光聚合水凝胶具有许多有利于软骨组织工程的特性，包括：能够在体内形成以填充复杂缺陷，具有潜在的粘合和集成特性，对聚合过程的空间和时间控制，以及生物信号很容易融入网络中控制细胞分化和组织形成。这些特性对于颅面重建中软骨的再生极其有益。考虑到这些好处，拟议研究的主要目标是： 。 合成并表征可通过酶降解过程再吸收的光聚合共聚物水凝胶网络， 。 研究封装在水凝胶网络中的软骨细胞形成的组织的数量、分布和类型作为水凝胶成分的函数， 。 将信号纳入水凝胶网络以控制光封装间充质干细胞的软骨分化，以及 。 分析这些技术临床应用所需的光聚合结构的关键问题。