Understanding structure and function of biomineralisation protein MmsFcc for green nanoparticle manufacture

了解用于绿色纳米粒子制造的生物矿化蛋白 MmsFcc 的结构和功能

• 批准号: 2453322
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2453322
• 关键词: Understanding; structure; function; biomineralisation; protein

Magnetic bacteria form precise magnetic nanoparticles (MNPs) in magnetosomes using magnetosome membrane specific (Mms) biomineralisation proteins.Mms proteins control nanomaterial formation in vitro so could be developed into additives for green nanomaterial manufacturing, if the process is better understood.The key drawbacks to realisation are:1. Mms membrane proteins are difficult to express/purify;2. Structural information is notoriously difficult to obtain, as Mms proteins are intrinsically disordered:- they have no crystal structures.The supervisors of this project collaborated to contribute significantly to understanding Mms using NMR but little is known structurally about more important Mms proteins like MmsF. Critically Dr Staniland has succeeded in overcoming drawback 1 by forming the scaffold construct MmsFcc and has formed a suite of point-mutated versions that have varying effect on MNP formation. Our hypothesis is structural features are critical to biomineralisation.This project will focus on characterising MmsFcc and mutants as they interact with iron ions and growing MNP using NMR and supported by other assays (e.g. ELISA), building up to assessing peptide sequences found from biopanning against MNPs, to form a hypothetical mechanism that can be tested with designed peptides and molecules with the hypothesized function. These could be optimised for green additives for nanomaterial manufacture.

磁细菌在磁体中形成精确的磁性纳米颗粒（MNP），使用磁化体膜特异性（MMS）生物矿化蛋白.MMS蛋白质控制纳米材料形成，因此可以将其发展成为绿色纳米材料制造的添加剂，以便于该过程更好地理解。 MMS膜蛋白很难表达/纯化； 2。众所周知，由于MMS蛋白质本质上是无序的： - 它们没有晶体结构。批判性的Staniland博士通过形成脚手架构造MMSFCC成功克服了缺点1，并形成了一套点突变版本，这些版本对MNP的形成具有不同的影响。 Our hypothesis is structural features are critical to biomineralisation.This project will focus on characterising MmsFcc and mutants as they interact with iron ions and growing MNP using NMR and supported by other assays (e.g. ELISA), building up to assessing peptide sequences found from biopanning against MNPs, to form a hypothetical mechanism that can be tested with designed peptides and molecules with the hypothesized function.这些可以针对纳米材料生产的绿色添加剂进行优化。