Comparative Genomics of Anopheline Mosquitoes

按蚊的比较基因组学

• 批准号: 6855392
• 负责人: Zhijian Jake Tu
• 金额: $ 23.86万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6855392
• 关键词: Anopheles; arthropod genetics; artificial chromosomes; behavioral genetics; biochemical evolution; chemoreceptors; disease vectors; functional /structural genomics; gene mutation; gene targeting; genetic mapping; genetic regulatory element; malaria; olfactions; species difference

DESCRIPTION (provided by applicant): Anopheline mosquitoes constitute the primary vectors of malaria. Recent developments in mosquito research, that were highlighted by the publication of a draft genome sequence of the primary African malaria vector Anopheles gambiae, provide exciting opportunities to greatly enhance our understanding of mosquito genetics, behavior, and physiology. New insights in mosquito biology will undoubtedly facilitate the development of novel strategies to combat malaria and other mosquito-borne diseases. The basic concept underlying this proposal is to combine comparative inquiry with modern genomic technology to help realize the promise of the An. gambiae genome project. The long-term objective of this exploratory R21 project is to develop an efficient method of multi-species comparisons of targeted genomic regions to illuminate what makes a mosquito a competent vector for pathogens, an anthropophilic biter, and an adaptive survivor under different ecological conditions. Here we will target genomic regions that contain odorant receptor (GPRor) genes or gene clusters because of their potential roles in host-seeking behavior of An. gambiae. We will pursue three Specific Aims: 1) Sequence 12 randomly picked BAC clones from An. stephensi to obtain baseline information on the relative genomic organization and evolutionary divergence between An. stephensi and An. gambiae. 2) Target 6 regions that contain GPRor genes or gene clusters for pair-wise comparisons between An. stephensi and An. gambiae to examine the evolutionary divergence, conserved regulatory elements, and expansions/loss/rearrangements of these genes. 3) Further target 2 GPRor regions for multi-species comparisons to efficiently identify potential regulatory elements, detect local gene expansions/loss/rearrangements, and examine possible correlations between these genetic changes and biological adaptations.

描述（由申请人提供）：蚊子蚊子构成疟疾的主要媒介。蚊子研究中的最新发展是由主要非洲疟疾媒介Anopheles Gambiae的基因组序列草案提出了强调的，这为我们提供了令人兴奋的机会，以极大地增强我们对蚊子遗传学，行为和生理学的理解。蚊子生物学的新见解无疑将有助于制定新的策略来打击疟疾和其他蚊子传播疾病。该提案的基本概念是将比较查询与现代基因组技术相结合，以帮助实现AN的希望。冈比亚基因组项目。该探索性R21项目的长期目标是开发一种有效的靶向基因组区域比较多种物种的方法，以阐明使蚊子成为病原体，拟人化剂量和适应性生存者在不同生态条件下的病原体媒介的原因。在这里，我们将针对包含气味受体（GPROR）基因或基因簇的基因组区域，因为它们在AN的宿主寻求行为中具有潜在的作用。冈比亚。我们将追求三个特定目标：1）序列12从An随机挑选的BAC克隆。斯蒂芬斯获得有关An之间的相对基因组组织和进化差异的基线信息。 Stephensi和An。冈比亚。 2）目标6个区域，其中包含Gpror基因或基因簇，以在An之间进行成对比较。 Stephensi和An。冈比亚检查这些基因的进化差异，保守的调节元件以及扩展/损失/重排。 3）进一步的目标2 Gpror区域进行多种物种比较，以有效识别潜在的调节元件，检测局部基因扩展/丢失/重排，并检查这些遗传变化与生物学适应之间的可能相关性。