Light-evoked inhibition at mouse bipolar cell terminals

小鼠双极细胞末端的光诱发抑制

• 批准号: 6881646
• 负责人: ERIKA D. EGGERS
• 金额: $ 4.83万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-12 至 2006-03-11
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6881646
• 关键词: GABA receptor; amacrine cells; axon; cell type; cellular polarity; cone cell; evoked potentials; gamma aminobutyrate; genetically modified animals; glycine; glycine receptors; laboratory mouse; neural information processing; neural inhibition; neural transmission; photostimulus; postdoctoral investigator; protein structure function; retina; rod cell; sectioning; synapses; tissue /cell preparation; visual stimulus; voltage /patch clamp

DESCRIPTION (provided by applicant): GABAergic and glycinergic inhibitory pathways in the inner plexiform layer modulate the flow of visual information between bipolar cells and ganglion cells and are an essential element in retinal information processing. In mammalian retina the precise roles of the different inhibitory neurotransmitters, receptor subtypes and circuits are not well understood. Different bipolar cell types separate the visual signal into distinct visual channels, e.g., rod versus cone, on versus off and sustained versus transient signals. Distinct GABAA and GABAC receptors found on bipolar cell terminals have been shown to have different biophysical properties and are present in different proportions on distinct cell types. Glycine receptors have also been shown to be present on bipolar cell terminals, but their functional roles on most bipolar cells is unclear. The goal of the proposed study is to analyze the role of GABAergic and glycinergic inhibition of bipolar cell terminals on spatial and temporal processing in the IPL. We will measure the light-evoked inhibitory signals in different bipolar cell types to determine whether these distinct receptor types mediate unique inhibitory signals and whether the distinct bipolar cell signaling pathways utilize distinct inhibitory signals, which affects transmission from bipolar cells to third order cells

描述（由申请人提供）：内丛状层中的GABA能和糖抑制途径调节双极细胞和神经节细胞之间的视觉信息流动，并且是视网膜信息处理中的重要元素。在哺乳动物视网膜中，不同抑制性神经递质的精确作用，受体亚型和电路尚不清楚。不同的双极单元类型将视觉信号分为不同的视觉通道，例如杆对锥，ON与OFF和持续信号与瞬态信号。在双极细胞末端发现的不同的GABAA和GABAC受体已显示出具有不同的生物物理特性，并且在不同的细胞类型上以不同的比例存在。甘氨酸受体也已显示在双极细胞末端，但它们在大多数双极细胞上的功能作用尚不清楚。拟议的研究的目的是分析双极细胞末端对IPL的空间和时间处理的GABA能和甘体能抑制的作用。我们将测量不同双极细胞类型中的光诱发抑制信号，以确定这些不同受体类型是否介导了独特的抑制性信号，以及不同的双极细胞信号传导途径是否利用了不同的抑制信号，这会影响从双极细胞到三阶细胞的传播