Susceptibility Testing Procedures for Rapidly Growing Mycobacteria and Nocardia

快速生长的分枝杆菌和诺卡氏菌的药敏试验程序

• 批准号: 6103691
• 负责人: Frank G Witebsky
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6103691
• 关键词: Actinomycetales; Mycobacterium; antibacterial agents; drug screening /evaluation; microorganism culture; microorganism growth

No standardized susceptibility testing procedures exist for rapidly growing mycobacteria or Nocardia species. Several different procedures have been suggested for use, including broth microdilution and the Etest (manufactured by AB Biodisk, Culver City, CA). We are comparing both a microdilution procedure (JustOne, manufactured by Radiometer America, Inc., Westlake, OH) and the Etest procedure, for their comparability and reproducibility, looking simultaneously at a number of other variables such as inoculum concentration, incubation time, and testing medium.The JustOne strips are useful because they allow selection of dilutions of individual antimicrobial agents that may be particularly relevant clinically, rather than the use of a whole array of agents as found in the usual microdilution plate format. We hope to be able to select a procedure that will provide rapid and reproducible results, and not be so technically demanding as to be unsuitable for use in the routine diagnostic laboratory. Work to date indicates that the Etest produces results that are difficult to interpret, and we intend to concentrate our further efforts on the microdilution procedure. We have also been involved in a multi-institution collaborative study to determine the inter- and intra-institution reproducibility of both the Etest and a microdilution procedure for susceptibility testing of rapidly growing mycobacteria. The inter-institution results have been collected and are currently being analyzed.

没有标准化的药敏试验程序 存在于快速生长的分枝杆菌或诺卡氏菌属物种中。一些 建议使用不同的程序，包括肉汤 微量稀释和 Etest（AB Biodisk、Culver 制造） 加利福尼亚州城市）。我们正在比较微量稀释程序 （JustOne，由位于 Westlake 的 Radiometer America, Inc. 制造， OH) 和 Etest 程序，因为它们具有可比性和 再现性，同时观察许多其他 接种浓度、培养时间等变量 测试介质。JustOne 试纸条很有用，因为它们允许 选择各种抗菌剂的稀释度 与临床特别相关，而不是使用整个阵列 常见微量稀释板形式中的试剂。我们希望 能够选择一个能够提供快速和 可重复的结果，并且技术要求不高 不适合在常规诊断实验室中使用。迄今为止的工作 表明 Etest 产生的结果很难 解释，我们打算将进一步的努力集中在 微量稀释程序。我们还参与了一个 多机构协作研究以确定相互之间和 Etest 和微量稀释的机构内重现性 快速生长的药敏试验程序 分枝杆菌。机构间结果已收集并 目前正在分析中。