SUSCEPTIBILITY TESTING PROCEDURES FOR RAPIDLY GROWING MYCOBACTERIA AND NOCARDIA

快速生长的分枝杆菌和诺卡氏菌的敏感性测试程序

• 批准号: 6289465
• 负责人: Frank G Witebsky
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6289465
• 关键词: Actinomycetales; Mycobacterium; antibacterial agents; drug screening /evaluation; microorganism culture; microorganism growth

No standardized susceptibility testing procedures exist for rapidly- growing mycobacteria or Nocardia species. Several different procedures have been suggested for use, including broth microdilution and the Etest (manufactured by AB Biodisk, Culver City, CA). The Etest produces results which are difficult to interpret with these organisms, and we do not plan to do any further work with this procedure for these organisms at present. We were collaborators in a multi-institution study to determine the inter- and intra-institution reproducibility of both the Etest and a microdilution procedure for susceptibility testing of rapidly-growing mycobacteria. The results of the microdilution procedure from this study have been analyzed, and the results have been published (Woods GL, et al, J. Clin. Microbiol. 1999;37:1676-1682). The microdilution procedure employed will probably become the reference procedure for susceptibility testing of rapidly-growing mycobacteria. This project is terminated.

对于快速生长的分枝杆菌或诺卡氏菌属物种，不存在标准化的药敏试验程序。已建议使用几种不同的程序，包括肉汤微量稀释和 Etest（由加利福尼亚州卡尔弗城的 AB Biodisk 制造）。 Etest 产生的结果很难用这些生物体来解释，我们目前不打算对这些生物体进行任何进一步的研究。我们是一项多机构研究的合作者，旨在确定用于快速生长的分枝杆菌敏感性测试的 Etest 和微量稀释程序的机构间和机构内重现性。本研究的微量稀释程序的结果已被分析，并且结果已发表（Woods GL 等人，J. Clin. Microbiol. 1999；37：1676-1682）。所采用的微量稀释程序可能会成为快速生长的分枝杆菌药敏试验的参考程序。该项目已终止。

项目成果

Multisite reproducibility of results obtained by the broth microdilution method for susceptibility testing of Mycobacterium abscessus, Mycobacterium chelonae, and Mycobacterium fortuitum.

通过肉汤微量稀释法对脓肿分枝杆菌、龟分枝杆菌和偶发分枝杆菌进行药敏试验获得的结果的多位点重现性。

• 发表时间: 1999-06
• 影响因子: 9.4
• 作者: Woods, G L;Bergmann, J S;Witebsky, F G;Fahle, G A;Wanger, A;Boulet, B;Plaunt, M;Brown, B A;Wallace Jr, R J
• 通讯作者: Wallace Jr, R J