Fluorous Solution-Phase Synthesis of Peptides

肽的氟溶液相合成

• 批准号: 6993221
• 负责人: NICOLA L. B. POHL
• 金额: $ 17.03万
• 依托单位: FLUOROUS TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6993221
• 关键词: carbopolycyclic compound; chemical synthesis; chromatography; glycolipids; glycopeptides; glycoproteins; glycosylation; method development; microarray technology; oligopeptides; oligosaccharides; carbopolycyclic compound; chemical synthesis; chromatography; glycolipids; glycopeptides; glycoproteins; glycosylation; method development; microarray technology; oligopeptides; oligosaccharides

DESCRIPTION (provided by applicant): Ready access to various oligosaccharides and glycopeptides is crucial for the development of glycobiology and glycomics-the system-wide study of the interactions of oligosaccharides with other biomolecules such as proteins. The long-term goal of the proposed project is to develop a new method for preparative scale syntheses of exceptionally pure oligopeptides, oligosaccharides, and glycopeptides utilizing Fluorous Technologies' proprietary tagging and purification techniques. The specific aims during Phase I of this STTR proposal are: 1) the development of fluorous-fluorenylmethoxycarbonyl (F-Fmoc) solution-phase oligopeptide synthesis (F-Fmoc Strategy); 2) the development of fluorous tag aided oligosaccharide synthesis; and 3) the development of fluorous tag aided oligosaccharide microarrays and multiwell-plate arrays. These developments will lay the foundation for the project's Phase II, which will include (1) parallel synthesis of oligosaccharide and glycopeptide libraries that have been reported to exhibit interesting biological activities such as immunogenicity against cancer cells, (2) development of automated solution-phase oligomer synthesis using a parallel synthesizer, a liquid handler, and an automated fluorous flash chromatography system, and (3) further exploration of fluorous tags for direct compound array production.

描述（由申请人提供）：准备进入各种寡糖和糖肽对糖脂学和糖基因的发展至关重要，这是对寡糖与其他生物分子（例如蛋白质）相互作用的系统范围研究。拟议项目的长期目标是开发一种新方法，用于制备异常纯寡肽，寡糖和糖肽的制备量表合成，利用易流技术的专有标记和纯化技术。在该STTR建议的第一阶段的特定目的是：1）开发荧光氟甲氧基碳苯子（F-FMOC）溶液相寡肽合成（F-FMOC策略）； 2）氟标签的发展辅助寡糖合成； 3）开发易流的标签辅助寡糖微阵列和多绿色板阵列。这些发展将为该项目的II阶段奠定基础，其中包括（1）平行合成寡糖和糖肽库的平行合成，据报道表现出有趣的生物学活性，例如针对癌细胞的免疫原性，（2）使用自动化溶液 - 相位寡素化合物的开发，并使用平行的液体构造，以及自动化的液体构造，以及3次，以及3个均匀的合成，以及3.直接复合阵列生产的荧光标签。