Technology for DNA Damage Analysis and Biomarkers
DNA 损伤分析和生物标记技术
基本信息
- 批准号:6622497
- 负责人:
- 金额:$ 13.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-05-15 至 2005-04-30
- 项目状态:已结题
- 来源:
- 关键词:DNA binding protein DNA damage affinity labeling antibody titering biomarker biotechnology capillary electrophoresis carcinogen testing chemical carcinogen clinical research environment related neoplasm /cancer environmental exposure experience fluorescent dye /probe green fluorescent proteins high throughput technology immunofluorescence technique lasers mixed tissue /cell culture monoclonal antibody neoplasm /cancer epidemiology nucleic acid probes nucleic acid quantitation /detection radiation carcinogen technology /technique development
项目摘要
DESCRIPTION (provided by applicant):
Characterization of environmental determinants of cancer is an important goal
for developing effective public health strategies in prevention and control of
cancer. Informative epidemiological investigations of environment-cancer
relationships rely on accurate, quantifiable exposure measurements. In
traditional population surveys that attempt to link a substance with cancer
occurrence, exposure data is often obtained indirectly in the form of
questionnaires or personal interviews. The uncertainty associated with errors
in exposure assessment has obscured or limited identification of cancer
etiologies. Rather than the current reliance on external dose estimation and
extrapolation from high dose exposure with rodent models, sufficiently
sensitive biomarkers based on DNA damage measurements could allow for a more
realistic assessment of environmental risk. A broad, long-term objective of
this project is to develop innovative, cost-effective, and reliable technology
for quantifying exposures, especially low level exposures. Specific aims
include developing highly sensitive technology for measuring minute amounts of
DNA damage and to improve biomarkers for epidemiological investigations of
environment-cancer relationships. The technology combines the specificity of
antibodies and capillary electrophoresis separation with high sensitivity of
laser induced fluorescence detection. In addition, specific DNA-recognition
proteins will be used to bind with various DNA lesions for detection of a
range of DNA lesions. Automated and high throughput assay formats making use
of the high sensitivity technology will be explored. The sensitive DNA damage
measurements will be used as potential biomarkers for exposure assessment. The
technology will also be useful for monitoring DNA damage induced by anticancer
agents and for studying DNA repair, which is an essential protection mechanism
against cancer initiation.
描述(由申请人提供):
癌症环境决定因素的表征是一个重要目标
制定有效的公共卫生策略来预防和控制
癌症。环境-癌症的信息流行病学调查
关系依赖于准确、可量化的暴露测量。在
试图将某种物质与癌症联系起来的传统人口调查
发生时,暴露数据通常以以下形式间接获得:
问卷调查或个人访谈。与错误相关的不确定性
暴露评估模糊或限制了癌症的识别
病因学。而不是目前依赖外部剂量估算和
从啮齿动物模型的高剂量暴露推断,充分
基于 DNA 损伤测量的敏感生物标记物可以提供更多
环境风险的现实评估。一个广泛的、长期的目标
该项目旨在开发创新、具有成本效益且可靠的技术
用于量化暴露,特别是低水平暴露。具体目标
包括开发高灵敏度技术来测量微量
DNA 损伤并改善流行病学调查的生物标志物
环境与癌症的关系。该技术结合了以下特性
抗体和毛细管电泳分离具有高灵敏度
激光诱导荧光检测。此外,特定的 DNA 识别
蛋白质将被用来与各种 DNA 损伤结合以检测
DNA 损伤范围。利用自动化和高通量测定格式
将探索高灵敏度技术。敏感DNA损伤
测量结果将用作暴露评估的潜在生物标志物。这
技术还可用于监测抗癌引起的 DNA 损伤
用于研究 DNA 修复(这是一种重要的保护机制)
对抗癌症的发生。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('X. Chris LE', 18)}}的其他基金
Technology for DNA Damage Analysis and Biomarkers
DNA 损伤分析和生物标记技术
- 批准号:
6769979 - 财政年份:2002
- 资助金额:
$ 13.5万 - 项目类别:
Technology for DNA Damage Analysis and Biomarkers
DNA 损伤分析和生物标记技术
- 批准号:
6448259 - 财政年份:2002
- 资助金额:
$ 13.5万 - 项目类别:
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