The role of TcpA in cholera immunity and vaccination

TcpA 在霍乱免疫和疫苗接种中的作用

• 批准号: 7025461
• 负责人: JASON B HARRIS
• 金额: $ 12.69万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-16 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7025461
• 关键词: B lymphocyte; Bangladesh; Vibrio cholerae; active immunization; attenuated microorganism; bacterial proteins; bactericidal immunity; biopsy; biotechnology; cholera; cholera vaccine; clinical research; disease /disorder model; human subject; immune response; laboratory mouse; live vaccine; protein structure function; vaccine development; vaccine evaluation

DESCRIPTION (provided by applicant): This application is for a 4-year International Research Scientist Development Award for the study of anti- bacterial immunity and vaccine development. The candidate will be under the mentorship of Dr. Stephen Calderwood and Dr. Edward Ryan at the Massachusetts General Hospital, Boston, Massachusetts, and Dr. Firdausi Qadri, at the ICDDR.B: Centre for Health and Population Research, Dhaka, Bangladesh (ICDDR.B). The candidate is currently completing a fellowship in Pediatric Infectious Diseases and plans to pursue an academic career studying enteric diseases of importance in developing countries. In the proposed program, the trainee will spend at least 50% of his time at the ICDDR.B. The training program will include courses and symposia focused on bacterial genetics, immunology and vaccine development. Hands-on experience will broaden the trainee's skills in the laboratory study of adaptive immunity, in the conduct and analysis of large observational studies in human subjects, and in the laboratory techniques necessary for translational research in vaccine development. The proposal is focused on improving understanding of the mechanisms of adaptive immunity to cholera. 3 specific aims of the project are: (1) to assess whether TcpA-specific immune responses are associated with protection from cholera; (2) to assess whether the Peru-15 live attenuated cholera vaccine induces comparable immune responses to natural cholera infection; and (3) to develop a transcutaneous vaccination strategy to boost TcpA-specific immune responses in an animal model of cholera. This research plan will allow the trainee to build on preliminary findings and to develop research skills related to vaccine development that can be used to support an independent research career. Relevance: This research will increase our understanding of immunity to cholera and may aid in the development of a cholera vaccine that could save thousands of lives each year in developing countries.

描述（由申请人提供）：本申请是为抗细菌免疫和疫苗开发研究的4年国际研究科学家发展奖。候选人将在马萨诸塞州波士顿，马萨诸塞州的马萨诸塞州综合医院的Stephen Calderwood博士和Edward Ryan博士的指导下，以及ICDDR.B的Firdausi Qadri博士：健康与人口研究中心，孟加拉国达卡，孟加拉国达卡（ICDDR.B）。该候选人目前正在完成小儿传染病的研究金，并计划从事发展中国家研究肠道疾病的学术职业。在拟议的计划中，学员将至少在ICDDR.B上花费50％的时间。该培训计划将包括关注细菌遗传学，免疫学和疫苗开发的课程和专题讨论会。实践经验将扩大受训者在适应性免疫的实验室研究，人类受试者的大型观察性研究的行为和分析以及疫苗开发转化研究所必需的实验室技术方面的技能。该提案的重点是提高对霍乱自适应免疫机制的理解。该项目的3个特定目的是：（1）评估TCPA特异性免疫反应是否与霍乱的保护有关； （2）评估秘鲁-15活疫苗疫苗是否会引起对天然霍乱感染的可比免疫反应； （3）制定经皮疫苗接种策略，以在霍乱动物模型中增强TCPA特异性免疫反应。该研究计划将使学员可以基于初步发现，并开发与疫苗开发有关的研究技能，该研究技能可用于支持独立的研究职业。相关性：这项研究将增加我们对霍乱免疫力的理解，并可能有助于开发霍乱疫苗，该疫苗每年可以在发展中国家挽救数千次生命。