In Vitro Robotic Assay for Estrogenic Activity

雌激素活性的体外机器人测定

• 批准号: 6740465
• 负责人: Cathy Z Yang
• 金额: $ 68.4万
• 依托单位: CERTICHEM, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6740465
• 关键词: MCF7 cell; antioxidants; biotechnology; cell proliferation; computer data analysis; diagnostic tests; endocrine disorder diagnosis; estrogens; food; high performance liquid chromatography; high throughput technology; neoplastic cell culture for noncancer research; nucleic acid quantitation /detection; plastics; robotics; technology /technique development

Recent scientific investigations have shown that many chemicals used in plastics, pharmaceuticals, )esticides, cosmetics, food additives, etc. are endocrine disruptors (EDs). EDs interfere in various ways with hormonal activity to have significant adverse effects on many physiological processes at very low (picomolar to nanomolar) concentrations, especially on fetal or developing mammals. The prevalence and actions of EDs in our environment warrants the development of highly specific, sensitive, reliable, rapid, and cost-effective detection. Consequently, various governmental bodies (EPA, FDA, and ICCVAM) and proactive corporations explicitly desire an in vitro robotic assay for EA, but no such assay is commercially available, much less validated. CertiChem (CCi) has just successfully completed a Phase I SBIR project to demonstrate that it is feasible to develop a robotic assay for EA using MCF-7 cell proliferation. In this Phase II application, CCi proposes to improve and extensively validate this assay so that it can be fully commercialized in Phase III. CCi also proposes to examine the relationship between theoretical predictions of EA for a set of antioxidants used in foodstuffs and plastics and the actual EA as measured by this robotic cell proliferation assay. Validation of our robotic screening assay for EA should be commercially important because of the large number of chemicals (>10,000) - much less chemical mixtures -that should be screened for EA by profit, non-profit, or governmental entities. Data from our robotic assay will also be of commercial importance because it will enable us to design proprietary formulations of additives that lack EA for foodstuffs and plastics.

最近的科学研究表明，塑料、药品、杀虫剂、化妆品、食品添加剂等中使用的许多化学物质都是内分泌干扰物（ED）。 ED 以多种方式干扰激素活性，在极低（皮摩尔至纳摩尔）浓度下对许多生理过程产生显着的不利影响，尤其是对胎儿或发育中的哺乳动物。我们环境中 ED 的流行和作用需要开发高度特异性、灵敏、可靠、快速且具有成本效益的检测方法。因此，各个政府机构（EPA、FDA 和 ICCVAM）和积极主动的公司明确希望对 EA 进行体外机器人测定，但此类测定尚未商业化 可用，更不用说经过验证了。 CertiChem (CCi) 刚刚成功完成了 I 期 SBIR 项目，以证明利用 MCF-7 细胞增殖开发 EA 机器人检测是可行的。在此 II 期应用中，CCi 建议改进并广泛验证该检测方法，以便其能够在 III 期中完全商业化。 CCi 还建议检查食品和塑料中使用的一组抗氧化剂的 EA 理论预测与通过机器人细胞增殖测定测量的实际 EA 之间的关系。 我们对 EA 的机器人筛选测定的验证应该具有重要的商业意义，因为营利性、非营利性或政府实体应筛选大量化学品 (>10,000)（更不用说化学混合物）来筛选 EA。我们的机器人检测数据也将具有商业重要性，因为它将使我们能够设计不含 EA 的食品和塑料添加剂专有配方。