An In Vitro Robotic Assay for Estrogenic Activity

雌激素活性的体外机器人测定

基本信息

批准号：
6446575
负责人：
Cathy Z Yang
金额：
$ 9.1万
依托单位：
CERTICHEM, INC.
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-03-01 至 2003-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6446575
关键词：
biosensor device biotechnology cell proliferation endocrine disorder estrogens high throughput technology hormone metabolism hormone regulation /control mechanism robotics technology /technique development

项目摘要

DESCRIPTION (provided by applicant): Recent scientific investigations have shown that many chemicals used in plastics, pharmaceuticals, pesticides, cosmetics, food additives, etc., are endocrine disruptors that interfere in various ways with the action of estrogen, androgen, or thyroid hormones. The most common endocrine effects of disruptor involve estrogenic activity that can have significant deleterious effects on many physiological processes at very low (picomolar to nanomolar) concentrations, especially on fetal or developing mammals. The prevalence and actions of endocrine disruptors in our environment warrants the development of highly specific, sensitive, reliable, rapid, and cost-effective detection. A high throughput robotic cell proliferation assay would constitute such a test. Hence, in this SBIR Phase I proposal, CertiChem proposes to develop a high throughput robotic assay to detect estrogenic activity by measuring in vitro proliferation of MCF-7 cells. Such a robotic screening assay should be commercially important because of the large number of chemicals (> 10,000) and mixtures of chemicals that should be screened for estrogenic activity by profit, non-profit, or governmental entities. Our preliminary data demonstrate that CertiChem has the expertise to develop this in vitro robotic assay for estrogenic activity. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（由申请人提供）：最近的科学研究表明表明许多化学品用于塑料、药品、农药、化妆品、食品添加剂等都是内分泌干扰物，会干扰通过多种方式与雌激素、雄激素或甲状腺激素作用。这干扰物最常见的内分泌效应涉及雌激素活性，可以对许多生理过程有显着的有害影响低（皮摩尔至纳摩尔）浓度，尤其是对胎儿或发育中的哺乳动物。我们环境中内分泌干扰物的流行和作用保证了高度特异、灵敏、可靠、快速和具有成本效益的检测。高通量机器人细胞增殖测定就构成了这样一个测试。因此，在本 SBIR 第一阶段提案中，CertiChem 提议开发一种高通量机器人检测来检测雌激素通过测量 MCF-7 细胞的体外增殖活性。这样的机器人筛选试验应该具有商业重要性，因为有大量的应筛查的化学品（> 10,000）和化学品混合物营利性、非营利性或政府实体的雌激素活动。我们的初步数据表明 CertiChem 拥有开发此产品的专业知识体外机器人雌激素活性测定。拟议的商业应用：不可用