Protein Characterization By Mass Spectrometry

蛋白质质谱表征

• 批准号: 6838404
• 负责人: Kenneth Tomer
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6838404
• 关键词: SDS polyacrylamide gel electrophoresis; mass spectrometry; matrix assisted laser desorption ionization; phosphoproteins; phosphorylation; posttranslational modifications; protein structure function; proteomics

As the human genome comes close to being completely identified, attention is turning to protein characterization, because the proteins are the substances actually involved in physiological processes, individually, posttranslationally modified and/or as part of complexes. State-of-the-art protein identification is based on mass spectrometric characterization of proteolytic peptides, either through peptide mapping data or through sequence tag data, combined with data base searching. In addition to protein identification, identification of post-translational modifications and sites of modification are extremely important aspects of protein characterization. Phosphorylation of proteins controls basic cellular processes and determination of the state of phosphorylation of a protein and the specific sites of phosphorylation provides important information that can be used for proposing mechanistic hypotheses, as well as for understanding the processes involved. We are actively developing and applying mass spectrometry-based methods for the characterization of phosphoproteins.

随着人类基因组几乎被完全鉴定出来，注意力正在转向蛋白质的特征，因为蛋白质是实际上与生理过程中实际相关的物质，单独，翻译后修饰和/或作为复合物的一部分。最先进的蛋白质识别基于蛋白水解肽的质谱表征，即通过肽映射数据或通过序列标签数据与数据库搜索结合使用。除了蛋白质鉴定外，翻译后修饰和修饰部位的鉴定是蛋白质表征的极为重要的方面。蛋白质的磷酸化控制基本的细胞过程并确定蛋白质的磷酸化状态和磷酸化的特定位点提供了重要信息，可用于提出机械假设，以及理解所涉及的过程。我们正在积极开发和应用基于质谱的方法来表征磷蛋白。