Quantitation Of Biomarkers Of Oxidative Stress By Nici-m

Nici-m 定量氧化应激生物标志物

• 批准号: 6677451
• 负责人: Kenneth Tomer
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6677451
• 关键词: alcoholic beverage consumption; antioxidants; biomarker; carcinogenesis; carotenoids; clinical research; eicosanoids; human data; human tissue; lipid peroxides; mass spectrometry; nutrition aspect of cancer; nutrition related tag; oxidative stress; peroxidation; prostaglandin analogs; tissue /cell culture

In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. Isoprostanes are prostaglandin-like compounds that are produced as a result of the chemical rather than enzymatic oxidation of arachidonic acid in membrane phospholipids. The isoprostanes have been shown to possess potent biological activity, and a number of studies have shown them to be accurate markers of lipid peroxidation in animal models of oxidative stress. The 8-iso-F2a-isoprostane has been the specific isomer quantified in most studies. The Mass Spectrometry Workgroup was asked to provide 8-iso-F2a-isoprostane analyses for NIEHS as part of the round-robin study of the various proposed biomarkers as well as to provide this capability to NIEHS scientists.

1996年，在NIEHS举行了有关人类氧化应激的测量研讨会。人们普遍的共识是，安装一项研究是有价值的，比较了从同一样品测量的氧化应激的各种标记。这样的标记是F2A-异丙烷，它由NICI-MS定量。异丙烷是前列腺素样化合物，是由于化学而非酶促氧化膜磷脂中产生的，而不是酶促氧化。异丙烷氏菌已显示具有有效的生物学活性，许多研究表明它们在氧化应激的动物模型中是脂质过氧化的准确标记。在大多数研究中，8-ISO-F2A-异丙烷已成为定量的特定异构体。要求质谱工作组为NIEHS提供8-ISO-F2A-异丙烷分析，作为各种提出的生物标志物的循环研究的一部分，并向NIEHS科学家提供了这种能力。