FORMATION OF THE DROSOPHILA SALIVARY GLAND

果蝇唾液腺的形成

• 批准号: 6497905
• 负责人: Deborah J Andrew
• 金额: $ 27.18万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497905
• 关键词: Drosophilidae; arthropod genetics; biological signal transduction; cell migration; developmental genetics; embryo /fetus tissue /cell culture; gene expression; gene mutation; gene targeting; genetic regulation; histogenesis; molecular cloning; mutant; protein protein interaction; protein structure function; salivary glands; transcription factor

DESCRIPTION (Adapted from the Investigator's Abstract): The long-term goal of this research is to understand in molecular detail how cells choose to form a particular organ and how they carry out this decision. The Principal Investigator proposes to address these questions by a systematic analysis of formation of a simple tissue, the salivary glands, in a genetically-tractable organism, the fruit fly, Drosophila melanogaster. In the past several years, the Principal Investigator has identified the factors that control where salivary glands will form and the number of cells committed to a salivary gland fate. In the next few years, the proposed studies will provide information on how these factors work in combination to control expression of salivary gland genes. The Principal Investigator also has perhaps the largest single collection of genes expressed in a specific tissue. Her goal is to pinpoint and characterize the genes that function very early in salivary gland development, particularly genes that coordinate the major morphogenetic movements required to internalize the salivary glands and to form functional epithelial tubes. To achieve these goals, four specific aims are proposed. In Aim 1, studies are proposed to test if activation of three early salivary gland genes by the transcription factors SCR, EXD and HTH is direct and, if so, will learn how TSH, ABD-B and DPP-signaling block activation. In Aim 2, studies are proposed to address the role of two early transcription factors, FKH and HKB, in salivary gland internalization. Fkh mutants fail to complete internalization of the salivary gland and show extensive salivary cell death. Hkb mutants initiate internalization at the wrong place and form abnormally shaped salivary glands. In Aim 3, studies are proposed to address the role of two genes, rib and D-SemaII, in positioning the salivary gland. Rib mutants have defects in directed migration of salivary gland cells and in D-SemaII mutants the distal portion of the salivary gland is abnormally positioned. In Aim 4, studies are proposed to find new genes required for salivary gland morphogenesis. These new genes will be identified both from the Principal Investigator's collection of genes known to be expressed in the developing salivary gland and from an EMS saturation screen. The proposed studies will provide a working blueprint for the early events of organ formation in all higher organisms.

描述（改编自研究者的摘要）：长期目标 这项研究旨在从分子细节上了解细胞如何选择形成 特定机构以及他们如何执行这一决定。校长 研究人员建议通过系统分析来解决这些问题 一种简单的组织——唾液腺——以基因可控的方式形成 有机体，果蝇，果蝇。在过去的几年里， 首席研究员已经确定了控制因素 唾液腺的形成以及唾液腺细胞的数量 命运。在未来几年中，拟议的研究将提供以下方面的信息： 这些因素如何结合起来控制唾液腺的表达 基因。首席研究员也可能拥有最大的单一 在特定组织中表达的基因的集合。她的目标是查明并 表征在唾液腺发育早期发挥作用的基因， 特别是协调所需的主要形态发生运动的基因 内化唾液腺并形成功能性上皮管。到 为实现这些目标，提出了四个具体目标。在目标 1 中，研究是 建议测试三个早期唾液腺基因是否被激活 转录因子 SCR、EXD 和 HTH 是直接的，如果是，将了解如何 TSH、ABD-B 和 DPP 信号传导阻断激活。目标 2 中提出了研究 解决两个早期转录因子 FKH 和 HKB 在 唾液腺内化。 Fkh突变体未能完成内化 唾液腺并显示出广泛的唾液细胞死亡。 HKb突变体发起 在错误的位置内化并形成形状异常的唾液腺。 在目标 3 中，建议研究解决两个基因 rib 和 D-SemaII，定位唾液腺。肋骨突变体有缺陷 唾液腺细胞的定向迁移，在 D-SemaII 突变体中，远端 唾液腺的一部分位置异常。在目标 4 中，研究是 提议寻找唾液腺形态发生所需的新基因。这些新 基因将从首席研究员的收集中被识别出来 已知在发育中的唾液腺和 EMS 中表达的基因 饱和度屏幕。拟议的研究将为 所有高等生物中器官形成的早期事件。