Analysis of LKP2 as a Plant Circadian Clock Regulator

LKP2 作为植物生物钟调节剂的分析

• 批准号: 6626208
• 负责人: HIEN G TRAN
• 金额: $ 4.16万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6626208
• 关键词: Arabidopsis; cell growth regulation; circadian rhythms; enzyme mechanism; gene expression; intermolecular interaction; molecular site; nonvisual photoreceptor; photoactivation; photobiology; plant genetics; plant growth /development; plant growth regulators; plant physiology; plant proteins; postdoctoral investigator; protein degradation; protein structure function; suppressor mutations; ubiquitin

DESCRIPTION (provided by applicant) The broad objective of this proposal is to contribute to the characterization of circadian rhythms in plants. By applying what is known about the mechanism, regulation, and influence of the plant clock to mammalian models, progress will be made toward the understanding and treatment of human circadian disorders. In Arabidopsis, genetic analyses have identified components of the plant circadian system. However, the function and position of these factors relative to the clock is not clear. One such factor, LKP2, has been implicated to act in the light input pathway and in proteolysis. The goal of this proposal is to ascertain the molecular activities of LKP2 and to determine how these actions affect the operation of the circadian clock. The specific aims of this proposal are: 1) investigate the role of LKP2 as a light sensor by examining chromophore binding and light regulation of protein stability/activity, 2) assess the involvement of LKP2 in ubiquitin-mediated protein degradation by assaying the functionality of the LKP2 F box, and 3) identify interacting proteins and genes via biochemical purification and by performing a suppressor screen. The findings of this study will assist in revealing the function and regulation of LKP2 as well as to uncover additional components of the plant circadian clock.

描述（由申请人提供） 该提案的总体目标是促进特征描述 植物的昼夜节律。通过应用对该机制的了解， 植物时钟对哺乳动物模型的调节和影响，进展将 旨在了解和治疗人类昼夜节律紊乱。在 拟南芥，遗传分析已确定植物昼夜节律的组成部分 系统。然而，这些因素相对于 时钟不清楚。其中一个因子 LKP2 被认为在 光输入途径和蛋白水解。该提案的目标是 确定 LKP2 的分子活性并确定这些作用如何 影响生物钟的运作。本提案的具体目标 是：1) 通过检查发色团来研究 LKP2 作为光传感器的作用 蛋白质稳定性/活性的结合和光调节，2) 评估 通过检测 LKP2 参与泛素介导的蛋白质降解 LKP2 F 盒的功能，以及 3) 识别相互作用的蛋白质和基因 通过生化纯化和进行抑制筛选。这 这项研究的结果将有助于揭示 LKP2 以及发现植物生物钟的其他组成部分。