Post-translational control mechanisms of the circadian clock

生物钟的翻译后控制机制

基本信息

批准号：
9115622
负责人：
DAVID E SOMERS
金额：
$ 31.14万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至 2019-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9115622
关键词：
Address Affect Arabidopsis Behavior Biochemical Biological Biological Assay Biological Models Cell Cycle Cell Nucleus Cells Chromatin Circadian Rhythms Clock protein Complex Cytosol Defect Developmental Process Environment Eukaryota Feedback Gatekeeping Gene Expression Gene Targeting Genes Genetic Genomics Goals Health Homodimerization Hour Human Imaging Techniques Individual Intracellular Transport Lead Link Mediating Messenger RNA Metabolism MicroRNAs Molecular Movement Mutate Mutation Nuclear Nuclear Pore Nuclear Pore Complex Organism Output Pathway interactions Peptide Hydrolases Phase Phenotype Phosphorylation Physiological Processes Plant Model Plants Post-Transcriptional Regulation Post-Translational Protein Processing Process Proteins RNA Processing Regulation Regulatory Pathway Role Running Series Site Slice Small RNA System Temperature Testing Time Yeasts base cellular imaging circadian pacemaker data mining feeding in vivo mutant novel nucleocytoplasmic transport plant growth/development programs tool tumor metabolism yeast two hybrid system

项目摘要

DESCRIPTION (provided by applicant): Circadian rhythms are nearly ubiquitous endogenous timing systems that help coordinate the myriad physiological, metabolic and developmental processes that occur continuously in each organism at all times of the day. This circadian clock is comprised at the molecular level of interlocked and autoregulatory feedback loops that are built from complex interactions that are constantly changing in relation to each throughout the 24 hour cycle. The long term goal of this project is to understand the functional relationships among the post- transcriptional processes that keep the circadian oscillator running. We are using genetic, genomic, biochemical and cell biological tools and strategies of the model plant Arabidopsis to identify the molecules and mechanisms that regulate the transport of important proteins and mRNA between the cytosol and nucleus. We are focused in part on how a range of post-translational modifications of clock proteins affect both their positional and temporal intracellular localization. Gatekeeping features of the intracellular environment will also be addressed, both on a genomic scale and at the level of a single molecular species. We are applying for the first time in circadian studies single cell imaging techniques using a photo switchable fluorescent protein to assess features of clock protein movement and turnover that will be applicable to non-plant circadian systems. We are also exploiting certain plant-specific advantages of small RNA processing to address other post- transcriptional control mechanisms of the circadian clock. Taken together our program will probe mechanisms of circadian control that should be broadly applicable across all eukaryotic systems.

描述（由申请人提供）：昼夜节律是几乎无处不在的内源性计时系统，有助于协调每个生物体在一天中的所有时间连续发生的无数生理、代谢和发育过程，该生物钟由连锁的分子水平组成。以及由复杂的相互作用构建的自动调节反馈循环，这些相互作用在整个 24 小时周期中不断变化。该项目的长期目标是了解转录后过程之间的功能关系。我们正在使用模式植物拟南芥的遗传、基因组、生化和细胞生物学工具和策略来识别调节细胞质和细胞核之间重要蛋白质和 mRNA 运输的分子和机制。部分涉及时钟蛋白的一系列翻译后修饰如何影响其细胞内位置和时间定位的特征，还将在基因组规模和单个分子物种水平上得到解决。我们首次在昼夜节律研究中应用单细胞成像技术，使用光可切换荧光蛋白来评估时钟蛋白运动和周转的特征，这将适用于非植物昼夜节律系统。我们还利用了某些植物特有的优势。小RNA处理以解决生物钟的其他转录后控制机制总而言之，我们的计划将探索广泛适用于所有真核系统的昼夜节律控制机制。