CYTOHISTOCHEMISTRY CORE

细胞组织化学核心

• 批准号: 6612276
• 负责人: DENIS G. BASKIN
• 金额: $ 18.28万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6612276
• 关键词: autoradiography; biomedical facility; cell morphology; clinical research; diabetes mellitus; electron microscopy; fluorescence microscopy; health science research support; histochemistry /cytochemistry; human tissue; image processing; immunocytochemistry; in situ hybridization; laser capture microdissection; photomicrography; tissue /cell preparation

Description (Provided by applicant) The Cytohistochemistry Core provides state-of-the-art technical support, facilities, and collaborative expertise for histochemical and morphological methods needed by DERC Affiliate Investigators. Cytohistochemistry Core services and facilities include tissue fixation, paraffin and cryostat tissue processing, histological and histochemical staining, immunocytochemistry, in situ hybridization, autoradiography, photomicrography and computer imaging, electron microscopy, and quantitative morphometric analysis. The Cytohistochemistry Core Director, Dr. Denis G. Baskin, is an established scientist in the fields of histochemistry and diabetes/endocrinology research. He is Research Professor of Medicine at the University of Washington and is also Senior Research Career Scientist in the VA Medical Research Service. The Core Director collaborates with DERC Affiliate Investigators and oversees the operations of the Core. In response to DERC Affiliate requests for electron microscopy Core support, the Cytohistochemistry Core recruited Dr. Thomas Wight to the position of Associate Core Director. Dr. Wight, a prominent cell biologist in the area of vascular biology and extracellular matrix, oversees the electron microscopy services of the Core, including a part-time technician. Charge backs are assessed to Core users to offset the cost of Core services. The Cytohistochemistry Core is a cost-efficient resource for a large group of DERC investigators and has been successful over the years in contributing to numerous publications by Affiliate Investigators. Going into the new funding period, the Cytohistochemistry Core has requests for service by 37 Affiliate Investigators for 40 projects, all with peer-reviewed grant support. The DERC also proposes to add a laser capture microdissection system to the Cytohistochemistry Core to provide Affiliates with the capability of obtaining microscopic samples of tissues and cells for molecular and biochemical analysis related to diabetes.

描述（由申请人提供） 细胞组织化学核心为DERC会员研究人员所需的组织化学和形态学方法提供了最先进的技术支持，设施和协作专业知识。细胞组织化学的核心服务和设施包括组织固定，石蜡和低温组织组织加工，组织学和组织化学染色，免疫细胞化学，原位杂交，自显影，照射图，计算机成像，计算机成像，电子显微镜以及定量形态分析。细胞组织化学核心主管Denis G. Baskin博士是组织化学和糖尿病/内分泌研究领域的知名科学家。他是华盛顿大学医学研究教授，还是VA医学研究服务的高级研究职业科学家。核心主任与DERC会员调查员合作，并监督核心的运作。为了响应DERC会员对电子显微镜核心支持的要求，细胞组织化学核心招募了Thomas Wight博士担任副总监的职位。怀特博士是血管生物学和细胞外基质领域的著名细胞生物学家，负责监督核心的电子显微镜服务，包括兼职技术员。对核心用户进行评估，以抵消核心服务的成本。细胞组织化学核心是一大批DERC研究人员的一种经济高效的资源，多年来，已成功为会员研究人员提供了众多出版物。进入新的融资期，细胞组织化学核心要求37个会员调查员要求40个项目提供服务，所有这些项目都得到了同行评审的赠款支持。 DERC还建议在细胞组织化学核心中添加激光捕获的显微解剖系统，以使分支机构能够获得与糖尿病有关的分子和生化分析的组织和细胞的微观样品。