CORE--MORPHOLOGY

核心--形态学

• 批准号: 6606520
• 负责人: DAWNA L ARMSTRONG
• 金额: $ 35.07万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6606520
• 关键词: autoradiography; biomedical facility; brain; cholinergic receptors; heart; histology; immunocytochemistry; in situ hybridization; laboratory mouse; morphology; mutant; nicotinic receptors; peripheral nervous system; postmortem; spinal cord; tissue /cell culture

The morphology core will provide a facility for the preparation and interpretation of the morphology (gross and microscopic) of the nicotinic acetylcholine receptor mutant animals to be investigated in this program project, i.e. the alpha3, alpha7, beta2, and alpha7L247T mutant animals, and possibly other mutant animals as they become relevant to the aims of the project. The animals will be examined at autopsy for gross anomalies, and individual organs, but particularly the brain, spinal cord, peripheral ganglia, and the heart will be studied using histology, histochemistry, immunocytochemistry, in situ hybridization, receptor autoradiography, and ultrastructural studies. The specialized work of the core, after completion of baseline studies of the mutant animals, will be requested by Drs. Dani, De Biasi, and Paylor when their projects raise specific questions pertaining to the localized chemoarchitecture of the heart, ganglia, and specific brain nuclei. The morphology core will be located at two sites, in Dr. Armstrong's research laboratory in the Feigin Center at Texas Children's Hospital, and in Dr. Jim Patrick's laboratory in the Division of Neuroscience in the Smith Research wing at Baylor College of Medicine. The two facilities are within 10 minutes walking distance, and are connected by email to other investigators of the Program Project and to each other. Dr. Armstrong will coordinate the studies in the two facilities and Dr. Broide will direct the studies, particularly the in-situ hybridization studies which will be carried out in the Patrick laboratory.

形态学核心将为烟碱乙酰胆碱受体突变动物的形态（毛和显微镜）的准备和解释提供一个设施，将在该程序项目中进行研究，即alpha3，alpha3，alpha7，alpha7，beta2和alpha7l247t突变动物，以及与其他相关动物相关的动物，这些动物与其他相关动物相关。将在尸检时检查这些动物的总异常和个别器官，尤其是大脑，脊髓，周围神经节和心脏，将使用组织学，组织化学，免疫细胞化学，原位杂交，受体足病学和超结构研究来研究心脏。核心的专门工作，在完成突变动物的基线研究后，将由Drs要求。当他们的项目提出与心脏，神经节和特定脑核有关的特定问题时，Dani，de Biasi和Paylor提出了特定的问题。形态核心将位于德克萨斯州儿童医院Feigin中心的阿姆斯特朗博士研究实验室，以及贝勒医学院史密斯研究局的神经科学部的吉姆·帕特里克（Jim Patrick）实验室。这两个设施在步行距离10分钟内，并通过电子邮件连接到该计划项目的其他调查人员以及彼此之间。阿姆斯特朗博士将协调这两个设施的研究，兄弟博士将指导研究，尤其是将在帕特里克实验室进行的原位杂交研究。