Strand Invasion of the Collagen Triple Helix

胶原三螺旋的链侵入

• 批准号: 6534538
• 负责人: JONATHAN A HODGES
• 金额: $ 4.37万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-31 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6534538
• 关键词: biomimetics; collagen; disulfide bond; fluorimetry; molecular assembly /self assembly; postdoctoral investigator; protein structure function; triple helix; wound healing

DESCRIPTION (provided by applicant): Single-stranded DNA can invade an existing DNA duplex to form a D-loop by displacing one parental strand and forming Watson-Crick base pairs to the other. The analogous process in the collagen triple helix represents an alternate method for the treatment of skin wounds, but it has not been observed. Single-stranded collagen peptides that invaded frayed collagen fibrils on opposite sides of a wound would, in effect, close the wound without the need for suturing. To develop this method, the ability of single-stranded peptides to invade the collagen triple helix will be assessed. A heterotrimeric, triple-helical collagen mimic templated by a cysteine knot will be constructed and added to a solution of single-stranded peptide consisting of tripeptide repeats known to form hyperstable triple helices. Binding of the single strands to the host will be assayed by fluorescence anisotropy and ultracentrifugation. To extend the effective reach of the single strands across a wound, multiple single-stranded peptides will be attached to the arms of a PAMAMdendrimer, and the ability of this multimeric structure to invade multiple collagen hosts will be assayed.

描述（由申请人提供）：单链 DNA 可以侵入现有的 DNA 双链体通过置换一条亲本链并形成 D 环 沃森-克里克碱基与另一个碱基配对。胶原蛋白中的类似过程 三螺旋代表了治疗皮肤伤口的另一种方法， 但尚未观察到。入侵的单链胶原蛋白肽 伤口两侧磨损的胶原纤维实际上会闭合 无需缝合伤口。为了开发这种方法，需要具备 将评估单链肽侵入胶原蛋白三螺旋的情况。 以半胱氨酸结为模板的异源三聚体、三螺旋胶原模拟物 将构建并添加到单链肽溶液中 由已知形成超稳定三螺旋的三肽重复组成。 通过荧光分析单链与宿主的结合 各向异性和超速离心。扩大单一有效范围 穿过伤口的链，多个单链肽将附着在 PAMAM 树枝状聚合物的臂，以及这种多聚体结构的能力 将检测侵入多种胶原宿主。