Synthesis and Characterization of Leukopolymersomes

无色聚合物囊泡的合成和表征

• 批准号: 6745978
• 负责人: JAMES A SILAS
• 金额: $ 4.73万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6745978
• 关键词: artificial membranes; biomaterial development /preparation; biomimetics; biophysics; cell cell interaction; cell surface receptors; colloids; copolymer; crosslink; integrins; leukocyte adhesion molecules; leukocytes; membrane model; membrane reconstitution /synthesis; molecular assembly /self assembly; postdoctoral investigator; receptor expression; selectins; statistics /biometry; vascular endothelium; vesicle /vacuole

DESCRIPTION (provided by applicant): The purpose of this project is to make block copolymer vesicles that reproduce the ability of leukocytes to interact and bind to endothelium. Leukocytes have the ability to traffic in the blood stream, yet bind firmly to cells in the area of inflammation or infection. By transplanting the molecules that govern these interactions onto the surface of a polymer vesicle, we hypothesize that the resulting polymer vesicles will interact and bind to model endothelium surfaces. The first aim is to create a polymer vesicle that will roll, or undergo a unique type of dynamic friction that is characteristic of selectin binding between neutrophils and endothelium. The second aim is to create a polymer vesicle that will both roll and firmly bind by modifying the surface to express receptors for both selectin and integrin binding. The ligands for selectin and integrin binding will be attached to the polymer vesicles by modifying the hydrophilic block of the polymer, poly(ethylene glycol), to react with either a primary amine terminated ligand, or through a hierarchical strategy that takes advantage of biotin-avidin binding and the availability of biotinylated ligands. The third aim of this project is to modulate the binding of leukocyte mimics through the manipulation of the polymer vesicle membrane. The degree of crosslinking and chemistry of the polymer membrane may be varied to reproduce or exceed the structure and strength typical of cell membranes.

描述（由申请人提供）：该项目的目的是制造块共聚物囊泡，以重现白细胞相互作用并结合到内皮的能力。白细胞具有在血液中交通的能力，但与炎症或感染区域的细胞牢固结合。通过将控制这些相互作用的分子移植到聚合物囊泡的表面上，我们假设所得的聚合物囊泡将相互作用并结合到模型内皮表面。第一个目的是创建一个将滚动或经历独特类型的动态摩擦的聚合物囊泡，这是中性粒细胞和内皮之间选择蛋白结合的特征的独特类型的摩擦。第二个目的是创建一个聚合物囊泡，该囊泡将通过修饰表面以表达Selectin和整联蛋白结合的受体来滚动和牢固结合。选择素和整联蛋白结合的配体将通过修饰聚合物聚合物聚合物聚（乙二醇）的亲水性嵌段，以与原代胺终止的配体反应，或通过利用生物素 - 维丁蛋白结合的生物素结合和生物素的可用性来反应。该项目的第三个目的是通过操纵聚合物囊泡膜来调节白细胞模拟物的结合。聚合物膜的交联和化学程度可能会变化，以繁殖或超过细胞膜的典型结构和强度。

项目成果

Adhesion of polymer vesicles.

• DOI: 10.1103/physrevlett.95.026101
• 发表时间: 2005-07
• 期刊: Physical review letters
• 影响因子: 8.6
• 作者: John J Lin;F. Bates;D. Hammer;J. Silas