CENTER FOR STRUCTURAL GENOMICS

结构基因组学中心

• 批准号: 6950547
• 负责人: Stephen Kevin Burley
• 金额: $ 6.3万
• 依托单位: SGX PHARMACEUTICALS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6950547
• 关键词: Escherichia coli; Internet; Saccharomyces cerevisiae; X ray crystallography; bacterial proteins; biomedical automation; biotechnology; computer program /software; computer system design /evaluation; cooperative study; data collection; data collection methodology /evaluation; functional /structural genomics; genetic library; high performance liquid chromatography; high throughput technology; information dissemination; mass spectrometry; model design /development; molecular biology information system; molecular cloning; protein purification; protein structure function; technology /technique development; ultraviolet spectrometry

DESCRIPTION (Taken from the Application): The Albert Einstein College of Medicine, the Department of Biology at Brookhaven National Laboratory, Mount Sinai School of Medicine, the Rockefeller University, and the Weill Medical College of Cornell University have formed the New York Structural Genomics Research Consortium (NYSGRC) for high-throughput, structural and functional studies of proteins. The research program takes the form of a Pilot Study focused on establishing a fully-integrated, high-throughput system for protein family classification and target selection, target protein expression, purification, biophysical characterization and crystallization, target protein structure determination by X-ray crystallography, and analyses and dissemination of the results. The long-term goal of the NYSGRC is to create an efficient, cost-effective Structural Genomics Center that will contribute substantially to the task of determining the 10,000 plus three-dimensional protein structures envisaged by the National Institute of General Medical Sciences Protein Structure Initiative. The specific aims of the NYSGRC application are: Aim 1. Identify target protein sequences for structural genomics. Aim 2. Develop high-throughput E. coli expression of soluble target proteins. Aim 3. Develop high-throughput production of target proteins. Aim 4. Develop high-throughput biophysical characterization of target proteins. Aim 5. Develop high-throughput crystallization of target proteins. Aim 6. Develop efficient experimental strategies for MAD crystallography. Aim 7. Develop high-throughput synchrotron data collection with target protein crystals. Aim 8. Develop and use an Internet-based computational pipeline for protein crystallography. Aim 9. Develop high-throughput molecular replacement tools for crystallography. Aim 10 Develop high-throughput comparative modeling for structural genomics. Aim 11. Develop efficient annotation and dissemination of protein structures and models. Aim 12. Develop an Internet-based "web-book" for use by the NYSGRC.

描述（取自申请）：阿尔伯特·爱因斯坦学院 医学，芒特布鲁克黑文国家实验室生物学系 西奈医学院，洛克菲勒大学和威尔医学 康奈尔大学学院已经形成了纽约的结构基因组学 用于高通量，结构和功能的研究联盟（NYSGRC） 蛋白质的研究。该研究计划采用试点研究的形式 专注于建立一个完全集成的高通量系统的蛋白质 家庭分类和目标选择，靶蛋白表达， 纯化，生物物理表征和结晶，靶蛋白 通过X射线晶体学确定结构，分析和分析和 结果传播。 NYSGRC的长期目标是创建一个 有效的，具有成本效益的结构基因组学中心，将有助于 基本上是确定10,000加三维的任务 国家通用医学研究所设想的蛋白质结构 科学蛋白结构倡议。 NYSGRC应用程序的具体目的是： 目标1。确定靶蛋白序列的结构基因组学。 目标2。发展可溶性靶蛋白的高通量大肠杆菌表达。 目标3。发展靶蛋白的高通量产生。 目标4。发展靶蛋白的高通量生物物理表征。 目标5。发展靶蛋白的高通量结晶。 目标6。为疯狂晶体学制定有效的实验策略。 目标7。与目标蛋白一起开发高通量同步加速器数据收集 晶体。 目标8。开发和使用基于Internet的蛋白质计算管道 晶体学。 目标9。开发高通量分子替换工具以进行晶体学。 AIM 10为结构基因组学开发高通量比较建模。 目标11。发展有效的注释和传播蛋白质结构 和模型。 AIM 12。开发基于Internet的“ Web-Book”，供Nysgrc使用。

项目成果

Comparative protein structure modeling using Modeller.

• DOI: 10.1002/0471140864.ps0209s50
• 发表时间: 2006-10-01
• 期刊: Current protocols in bioinformatics
• 作者: Eswar, Narayanan;Webb, Ben;Sali, Andrej
• 通讯作者: Sali, Andrej

Alternative splicing of mouse transcription factors affects their DNA-binding domain architecture and is tissue specific.

• DOI: 10.1186/gb-2004-5-10-r75
• 发表时间: 2004
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Taneri B;Snyder B;Novoradovsky A;Gaasterland T
• 通讯作者: Gaasterland T

Radiation-induced site-specific damage of mercury derivatives: phasing and implications.

辐射引起的汞衍生物的特定部位损伤：阶段和影响。

• DOI: 10.1107/s0907444905022316
• 发表时间: 2005
• 期刊: Acta crystallographica. Section D, Biological crystallography
• 作者: Ramagopal,UdupiA;Dauter,Zbigniew;Thirumuruhan,Radhakannan;Fedorov,Elena;Almo,StevenC
• 通讯作者: Almo,StevenC

Structure of Escherichia coli YfdW, a type III CoA transferase.

• DOI: 10.1107/s0907444904000034
• 发表时间: 2004
• 期刊: Acta crystallographica. Section D, Biological crystallography
• 作者: A. Gogos;J. Gorman;L. Shapiro

Crystal structure of the Escherichia coli SbmC protein that protects cells from the DNA replication inhibitor microcin B17.

大肠杆菌 SbmC 蛋白的晶体结构，可保护细胞免受 DNA 复制抑制剂小菌素 B17 的侵害。

• DOI: 10.1002/prot.10102
• 发表时间: 2002
• 期刊: Proteins.
• 作者: Romanowski,MichaelJ;Gibney,SalleyA;Burley,StephenK
• 通讯作者: Burley,StephenK