Core D: Bone Cell Core

核心D：骨细胞核心

• 批准号: 6774670
• 负责人: MARK C HOROWITZ
• 金额: $ 12.54万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6774670
• 关键词: biomedical facility; bone; bone marrow; densitometry; embryo /fetus; laboratory mouse; laboratory rabbit; laboratory rat; mesenchyme; mixed tissue /cell culture; musculoskeletal disorder; osteoblasts; osteoclasts; pluripotent stem cells; tissue /organ preservation; training; biomedical facility; bone; bone marrow; densitometry; embryo /fetus; laboratory mouse; laboratory rabbit; laboratory rat; mesenchyme; mixed tissue /cell culture; musculoskeletal disorder; osteoblasts; osteoclasts; pluripotent stem cells; tissue /organ preservation; training

Although standard techniques are available, intra-laboratory variation in the quality and number of bone cells available for research purposes at Yale has varied considerably. To address this limitation and to advance productive scholarship, the Yale Core Center for Musculoskeletal Disorders developed the Cell Core. The mission of the Cell Core is to facilitate the experimental use of authentic, primary, mouse and rat osteoblastic cells as well as osteoclasts generated from in vitro culture. As part of our new initiatives bone marrow derived mesenchymal stem cells (MSCs) are now available to Core investigators. Although outside the original mandate of the Core, the Director has made his PIXImus machine available to Center members through an agreement with the Densitometry subunit of the Physiology Core. The Specific Aims of the current proposal are to provide the following services: 1. Isolate and cultivate primary murine and fetal rat calvarial osteoblasts. This will include large-scale preparations for biochemical studies and RNA and DNA purification. 2. Cultivate murine osteoclast-like cells generated from: 1) in vitro co-culture 2) culture with M-CSF and RANKL. 3. Maintain a wide range of frozen cell lines. 4. Provide small animal bone densitometry (DEXA). 5. Cultivate mouse bone marrow derived mesenchymal stem cells (MSCs). 6. Train for in vitro isolation and culture of osteoblasts, osteoclasts and stromal cells, so that investigators, appropriately trained, can initiate and maintain these cells in their laboratories. The Cell Core is particularly anxious to support current and future Pilot and Feasibility Projects as part of the Center's ongoing effort to attract new investigators to musculoskeletal research.

尽管有标准技术可用，但质量内实验室的变化 耶鲁大学可用于研究目的的骨细胞数量差异很大。到 解决这一限制并提高生产奖学金，耶鲁大学核心中心 肌肉骨骼疾病发展了细胞核。细胞核的任务是 还促进实验，原代，小鼠和大鼠成骨细胞的实验使用 正如体外培养产生的破骨细胞。 作为我们新计划的一部分，骨髓衍生的间充质干细胞（MSC）现在已 可供核心调查员。虽然超出了核心的原始任务，但 董事已通过协议使他的Piximus机器可用于中心成员 具有生理核心的光密度测量亚基。 当前建议的具体目的是提供以下服务： 1。分离和培养原发性鼠和胎儿大鼠钙细胞成骨细胞。这将包括 用于生化研究以及RNA和DNA纯化的大规模准备。 2。培养从以下几点培养以下的鼠破骨细胞样细胞：1）体外共培养2）与M-CSF和RANKL培养。 3。保持各种冷冻细胞系。 4。提供小动物骨密度测定法（DEXA）。 5。培养小鼠骨髓衍生的间充质干细胞（MSC）。 6。用于体外隔离和成骨细胞，骨细胞和基质细胞的培训，以便 经过适当培训的研究人员可以在实验室中启动和维护这些细胞。 该细胞核心特别渴望支持当前和未来的飞行员和可行性项目，这是该中心正在努力吸引新研究人员进行肌肉骨骼研究的努力的一部分。