CORE--BASIC GENOMICS AND PROTEOMICS FACILITY

核心——基础基因组学和蛋白质组学设施

• 批准号: 6989514
• 负责人: Robert Edward Ferrell
• 金额: $ 12.1万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989514
• 关键词: biomedical facility; cytogenetics; functional /structural genomics; genetic manipulation; genetic techniques; genome; microarray technology; neoplasm /cancer genetics; nucleic acid quantitation /detection; nucleic acid sequence; polymerase chain reaction; proteomics; serial analysis of gene expression; single nucleotide polymorphism

The Basic Genomics and Proteomics Facility(BGPF) is a newly proposed core to provide state-of-the-art genomic and proteomic technologies to UPCI investigators for basic research applications. The services encompass a wide range of technologies including cytogenetics, DNA sequencing, DNA and RNA isolation, Serial Analysis of Gene Expression (SAGE) library production, SNP genotyping, STRP genotyping, Array analysis (Affymetrix, CodeLink, and custom arrays), Quantitative RT-PCR, Difference Gel Electrophoresis, Ciphergen Proteinchip Profiling, Protein identification and sequencing. The specific aims of the BGPF are: 1. To provide access to genomics technologies for basic cancer research and help investigators determine which technologies are most applicable for their research. 2. To provide access to proteomics technologies for basic cancer research and help investigators determine which technologies are most applicable for their research. 3. To provide access to tools and expertise in genomic and proteomic data analysis and interpretation.

基础基因组学和蛋白质组学设施 (BGPF) 是一个新提出的核心，旨在为 UPCI 研究人员提供最先进的基因组学和蛋白质组学技术，用于基础研究应用。服务涵盖广泛的技术，包括细胞遗传学、DNA 测序、DNA 和 RNA 分离、基因表达序列分析 (SAGE) 文库生产、SNP 基因分型、STRP 基因分型、芯片分析（Affymetrix、CodeLink 和定制芯片）、定量 RT -PCR、差异凝胶电泳、Ciphergen 蛋白质芯片分析、蛋白质鉴定和测序。 BGPF 的具体目标是： 1. 为基础癌症研究提供基因组学技术，并帮助研究人员确定哪些技术最适用于他们的研究。 2. 为基础癌症研究提供蛋白质组学技术，并帮助研究人员确定哪些技术最适用于他们的研究。 3. 提供基因组和蛋白质组数据分析和解释方面的工具和专业知识。