Acquiring robotic support for mass spectrometry core

获得质谱核心的机器人支持

• 批准号: 6580590
• 负责人: CRAIG C MALBON
• 金额: $ 11.9万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580590
• 关键词: biomedical equipment purchase; high throughput technology; mass spectrometry; proteomics; robotics; biomedical equipment purchase; high throughput technology; mass spectrometry; proteomics; robotics

DESCRIPTION (provided by applicant): The Center for Analysis & Synthesis of Marcomolecules (CASM) at Stony Brook requests funding to acquire a robotic workstation for proteomics to process gel plugs for analysis by mass spectrometry. The institution has acquired an ABI Voyager research-grade MALDI mass spectrometer and a Micromass QToF tandem mass spectrometer to support proteomics research at Stony Brook. The present application seeks support for the acquisition of a Micromass MassPREP workstation to provide an automated, 96-well format digestion and spotting/sampling of gel plugs for detailed mass spectrometry. There are five major users who need the high-throughput capacity to enable their NIH-funded research. Dr. Danial Bogenhagen (Pharmacology) will employ the instrumentation in mitochondrial proteomics, an area of high interest, seeking to define the full complement of proteins that act in maintenance of mtDNA. Dr. Wen-Tien Chen (Medicine-Oncology) requires the instrumentation to enable his work on the proteomics of invasive cell protrusions involved in angiogenesis and metastasis. Dr. Malbon (Pharmacology) is using proteomics to deduce the composition of large macromolecular signaling complexes for G-protein-coupled receptors as well as in beta-catenin/Lef-Tcf pathways. Todd Miller (Physiology) requires this instrumentation in his proteomic analysis of tyrosine kinases, focusing upon the SH3-dependent partners of Hck and interacting protein partners for Jak2. William Van Nostrand (Medicine-Vascular Biology) is probing the processing of amyloid beta-protein precursor as well as the identification of new protein-protein interactions using proetomics. All of these investigators have published research based in protein chemistry and in mass spectrometry. To optimize the use of the instrumentation, the new workstation will be located in CASM, a protein core facility with more than a decade of experience. Three FTEs have been recruited to manage the mass spectrometry employed in proteomics. The institutional commitment includes the on-going support for these 3 FTEs as well as provision of funds to acquire a complementary automated gel scanner/spot picker jointly by the School of Medicine and the Vice-President for Research. The research proposals are high quality, well-funded by the NIH, and will benefit by the addition of this instrumentation. The institution has provided resources to enable the acquisition of two new mass spectrometers and this proposal builds on the investment.

描述（由申请人提供）：Stony Brook在Stony Brook要求的Marcomolecules（CASM）的分析与合成中心请求资金，以获取用于蛋白质组学的机器人工作站以处理凝胶插头，以通过质谱法进行分析。该机构已经获得了ABI Voyager研究级MALDI质谱仪和Micromass QTOF串联质谱仪，以支持Stony Brook的蛋白质组学研究。本应用程序寻求支持获取Micromass MassPrep工作站，以提供自动化的96孔格式消化和凝胶塞的斑点/采样，以详细质谱。有五个主要用户需要高通量能力来实现其NIH资助的研究。 Danial Bogenhagen博士（药理学）将在感兴趣的线粒体蛋白质组学中使用仪器，以寻求定义在维持mtDNA中作用的蛋白质的完整补体。 Wen-Tien Chen博士（医学 - 综合学）要求该仪器能够在涉及血管生成和转移涉及的侵入性细胞突起的蛋白质组学方面的工作。 Malbon博士（药理学）正在使用蛋白质组学来推断G蛋白偶联受体以及β-Catenin/Lef-TCF途径中的大型大分子信号传导复合物的组成。托德·米勒（Todd Miller）（生理学）在其对酪氨酸激酶的蛋白质组学分析中需要这种仪器，重点是HCK的SH3依赖性伴侣和JAK2相互作用的蛋白质伴侣。威廉·范·诺斯特兰（William van Nostrand）（药物血管生物学）正在探测淀粉样蛋白β-蛋白质前体的加工以及使用protomics的新蛋白质蛋白质相互作用的鉴定。所有这些研究者都发表了基于蛋白质化学和质谱法的研究。为了优化仪器的使用，新的工作站将位于CASM，这是一种拥有十多年经验的蛋白质核心设施。已经招募了三个FTE来管理蛋白质组学采用的质谱。机构承诺包括对这三个FTE的持续支持以及提供资金，以共同获得医学院和研究副主席的互补自动凝胶扫描仪/现货选择器。研究建议是高质量的，由NIH资金充足，并将通过增加该仪器而受益。该机构提供了资源来使两种新的质谱仪获得收购，该提案以投资为基础。