MoFlo High Speed Multilaser Flow Cytometer/Cell Sorter

MoFlo 高速多激光流式细胞仪/细胞分选仪

• 批准号: 6581673
• 负责人: Charles L. Goolsby
• 金额: $ 44.08万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581673
• 关键词: biomedical equipment purchase; cell sorting; flow cytometry; technology /technique development

DESCRIPTION (provided by applicant): Flow cytometry has become a key and critical research tool with applications in a wide range of disciplines in basic research, clinical translational research, and clinical studies. A wealth of reagents to identify specific cell sub-population, to study molecules regulating cell proliferation, apoptosis, signal transduction pathways, and functional states in cells are now available. Further, the fluorescent dyes and labeling techniques are now available to permit the simultaneous detection of many of these molecules and markers in individual cells. This is leading to the need for increasingly complex cytometric assays to understand the role and interaction of these molecules at the cell level. The Flow Cytometry Core Facility of the Robert H. Lurie Comprehensive Cancer Center at Northwestern University serves over 59 investigators with national peer reviewed funding of $22,631,071. This facility has a strong history of close, active interaction with its user base facilitating implementation of more difficult flow cytometry assays by all investigators regardless of their level of expertise. This coupled with the increase in available reagents has fostered increasingly complex requests of the facility. At present, the facility has a bench top analyzer, a Beckman-Coulter XLMLC four color instrument and a Beckman-Coulter ELITE ESP cell sorter which is over six years old. The current cell sorter has distinct limitations in the areas of complex, multi-laser and high speed sorting applications. Key for the Flow Cytometry Core Facility to meet the current and future needs of our user base is development of more complex and sophisticated multi-parameter and high-speed sorting capabilities. The requested MoFlo(TM) instrumentation will facilitate this critical development. This development will be aided by the MoFlo's enhanced multi-laser capabilities (three separate laser interrogation spots, focused/pinhole collection for all fluorescence detectors) and high speed sorting capabilities (25,000-50,000 cells/sec, 10-15X our current instrument capabilities). Further, the facilities oversight by Director, Dr. Charles Goolsby, with over 23 years experience in research and clinical flow cytometry and Mary Paniagua MT(ASCP)SI, with over 12 years experience in flow cytometry ensure that efficient implementation of these enhanced capabilities will occur.

描述（由申请人提供）：流式细胞术已成为基础研究，临床翻译研究和临床研究中广泛学科的关键和关键研究工具。现在可以使用大量试剂来鉴定特定的细胞亚群，以研究调节细胞增殖，凋亡，信号转导途径和功能状态的分子。此外，现在可以使用荧光染料和标记技术，以同时检测单个细胞中的许多这些分子和标记。这导致需要越来越复杂的细胞图测定来了解这些分子在细胞水平上的作用和相互作用。西北大学Robert H. Lurie综合癌症中心的流式细胞仪核心设施为59多名调查员提供服务，全国同行审查了22,631,071美元的资金。该设施与用户群促进了所有研究人员对更困难的流式细胞仪分析的实施，无论其专业水平如何，该设施都具有牢固的积极互动。再加上可用试剂的增加，它促进了该设施日益复杂的要求。目前，该设施配备了台式分析仪，贝克曼 - 库尔特XLMLC四种彩色仪器和贝克曼 - 库尔特精英ESP分隔器已有六年以上的历史。当前的细胞分辨率在复杂，多激光和高速分类应用领域具有不同的局限性。流式细胞仪核心功能以满足用户群的当前和未来需求的关键是开发更复杂和复杂的多参数和高速分类功能。请求的MOFLO（TM）仪器将促进这一关键发展。 MOFLO的增强多激光功能（三个独立的激光询问点，所有荧光探测器的聚焦/针孔收集）和高速分类功能（25,000-50,000单元/秒，10-15x，10-15x我们当前的仪器功能）将有助于这种发展。此外，主任查尔斯·古尔斯比（Charles Goolsby）博士的设施监督在研究和临床流式细胞仪方面拥有超过23年的经验，而玛丽·帕尼亚加（Mary Paniagua）MT（ASCP）SI则具有超过12年的流式细胞仪经验，可确保有效实施这些增强功能。