Laser Capture Microdissection Instrument

激光捕获显微切割仪

• 批准号: 6582556
• 负责人: Anthony E Oro
• 金额: $ 13.47万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6582556
• 关键词: biomedical equipment purchase; cell type; histogenesis; laser capture microdissection

DESCRIPTION (provided by applicant): The principal objective of this proposal is to provide access to a Laser Capture Microdissection system to investigators in the Program in Epithelial Biology. The Program in Epithelial Biology at Stanford provides a research forum for investigators with a common interest in mechanisms and diseases involving epithelial tissues. While each of the investigators uses sensitive techniques to determine gene expression differences within identified cell populations, a rate-limiting step in analysis is the ability to rapidly isolate and analyze pure populations of cells from tissue. Laser Capture Microdissection is a technique developed in 1996 that enables investigators to acquire individual groups of cells from tissue sections, fixed cell cultures and cytotogical preparations. The PixCell lle Microdissection Instrument from Arcturus Inc is currently the best system to perform the technique. The addition of a Laser Capture Microdissection system will significantly extend the research capabilities of the investigators in this program. The specific protocols presented in this proposal address fundamental mechanisms in epithelial growth and morphogenesis that will further our understanding of disease processes involved in athlerosclerosis, non-melanoma skin cancer, tumor metastasis, and axonal regeneration.

描述（由申请人提供）：该提案的主要目的是向上皮生物学计划中的研究人员提供对激光捕获的微解剖系统的访问。斯坦福大学上皮生物学的计划为对涉及上皮组织的机制和疾病的共同兴趣的研究人员提供了一个研究论坛。虽然每个研究人员使用敏感技术来确定已识别的细胞群体中的基因表达差异，但分析的限制步骤是能够快速分离和分析细胞与组织的纯群体。激光捕获显微解剖是一种于1996年开发的一种技术，它使研究人员能够从组织切片，固定细胞培养物和细胞逻辑制剂中获取单个细胞组。 Arcturus Inc的PixCell LLE LLE微解剖仪器目前是执行该技术的最佳系统。激光捕获显微解剖系统的添加将显着扩展该计划中研究人员的研究能力。本提案中提出的特定方案介绍了上皮生长和形态发生中的基本机制，这将进一步了解我们对涉及疾病过程，非黑色素瘤皮肤癌，肿瘤转移和轴突再生的理解。