Transmission Electron Microscopy Instrumentation

透射电子显微镜仪器

• 批准号: 6730833
• 负责人: MARK S MOOSEKER
• 金额: $ 38.01万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730833
• 关键词: biochemistry; biomedical equipment purchase; cytoskeleton; organelles; transmission electron microscopy

DESCRIPTION (provided by applicant): Ultrastructural analyses using a variety of experimental approaches are central to ongoing and proposed studies within the laboratories of the primary user groups participating in this shared instrumentation proposal. The primary user group consists of five, highly interactive, NIH-funded laboratories (Mooseker, Forscher, Pollard, Rosenbaum and Wells), all of which are members of the Department of Molecular, Cellular and Developmental Biology (MCDB), Yale University. The long term objective of this proposal is to upgrade/replace the transmission electron microscopy (TEM) equipment currently housed in the MCDB EM facility. This well established facility, maintained and operated by a full time, highly skilled EM technician/instructor, provides both research and training support to the research laboratories in MCDB. The principal investigator and four co-investigators represent the current major research groups using this facility, although a number of other laboratories from several departments also use this facility. Unfortunately, the existing TEMs and ancillary support equipment in this facility are quite old and maintenance due to lack of replacement parts will soon render this facility inoperable. As a consequence, the NIH-funded research projects of the user group, all of which employ ultrastructural analyses, are in jeopardy. The research goals of the user group all focus on structural, biochemical and functional analyses of a wide range of components of the eukaryotic cell cytoskeleton. TEM is used for a broad scope of investigations, from ultrastructural studies on purified cytoskeletal (both actin-filament and microtubule associated) components, isolated organelles, cells and tissues, including cells with altered cytoskeletal functions resulting from mutations in various cytoskeletal components. The requested TEM equipment is essential for the research objectives of the user group, and will also provide much needed research support for the broader research community at Yale.

描述（申请人提供）：使用多种实验方法进行的超微结构分析对于参与此共享仪器建议的主要用户群体的实验室中正在进行的和拟议的研究至关重要。主要用户组由五个，高度互动，NIH资助的实验室（Mooseker，Forscher，Pollard，Rosenbaum和Wells）组成，所有这些都是分子，细胞和发育生物学系（MCDB）的成员，耶鲁大学。该提案的长期目标是升级/更换MCDB EM设施中目前包含的传输电子显微镜（TEM）设备。这个由全职技术人员/讲师全职维护和运营的设施良好的设施为MCDB的研究实验室提供了研究和培训支持。主要研究人员和四名共同研究人员代表了目前使用该设施的主要研究小组，尽管来自多个部门的许多其他实验室也使用了此设施。不幸的是，该设施中现有的TEMS和辅助支持设备非常古老，并且由于缺乏替换零件而维护，很快就会使该设施无法使用。结果，用户组的NIH资助的研究项目（所有这些都采用了超微结构分析）都处于危险之中。用户组的研究目标都集中在真核细胞细胞骨架的各种成分的结构，生化和功能分析上。 TEM用于广泛的研究范围，来自对纯化细胞骨架（肌动蛋白丝和微管相关）组件的超微结构研究，分离的细胞器，细胞和组织，包括细胞骨架功能改变的细胞，这些细胞因各种细胞骨架成分的突变而导致的细胞骨架功能改变。要求的TEM设备对于用户组的研究目标至关重要，还将为耶鲁大学更广泛的研究社区提供急需的研究支持。