HIGH-SPEED MICROFLUIDIC SORTING OF BIOHAZARDOUS CELLS.

生物危害细胞的高速微流体分选。

• 批准号: 6793270
• 负责人: CHRISTOPHER J. FREDERICKSON
• 金额: $ 10.63万
• 依托单位: NEUROBIOTEX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6793270
• 关键词: biohazard detection; biomedical equipment development; bioterrorism /chemical warfare; cell line; computer data analysis; cytotoxicity; flow cytometry; fluid flow; high throughput technology

DESCRIPTION (provided by applicant): Effective National Defense against bio-terrorism demands safe, rapid identification of the pathogens used by terrorists. We will build an inexpensive, table top, cell sorter (flow cytometer) for rapid, safe processing of bio-hazardous cells and materials in the Bio-safety Level-4 environment. (BSL-4). Unlike other "Iab-on-a-chip" designs, ours promises ultra-high-throughput, due to our unique, (patent pending) massively series-parallel design. We estimate we can separate single rare cells from background populations up to 1,OOO-fold faster than current designs allow. The key to our miniature and massively series-parallel design is that we have eliminated the complex, bulky lasers and photo-multiplier detector equipment of today's cell sorter. Instead, we will use cheap semiconductor light sources, such as LED's, diode lasers, or VCELS, and semiconductor photo-detectors, such as photodiodes, diode arrays, and CCD's. Indeed, with filter coating applied to small pixel clusters, a single CCD can analyze an entire emission spectrum. In Phase I we will evaluate and select among competing micro-photonic and micro-fluidic (valving) approaches. We will also build a bench-model prototype sorter and demonstrate 3 stages of series-parallel II sorting. In Phase ", we will build a multistage prototype for testing by Dr. C.J. Peters' team in the UTMB SL-4 laboratory This provides us a unique opportunity to obtain an accurate bio-safety testing system as applied to bio-defense applications, including CDC A-Level pathogens. Commercial Opportunities: Our sealed, portable, and ultra-high throughput sorter will find broad use in bio-defense and also in HTP sorting of cells (or beads, or particles) for stem cell therapy, genomics, proteomics, and combinatorial chemistry applications.

描述（由申请人提供）： 对生物恐怖主义的有效国防要求安全，快速识别恐怖分子使用的病原体。我们将建立一个廉价的桌面，细胞分系仪（流式细胞仪），以在生物安全级别4环境中快速，安全地处理生物危害细胞和材料。 （BSL-4）。与其他“芯片上的iab-on-a-chip”设计不同，由于我们独特的（专利待处理）大规模串联设计，我们承诺要超高通量。我们估计，我们可以将单个稀有细胞与后台种群分离到1个，速度比当前设计允许的速度快。 我们的微型和大规模串联设计的关键是，我们消除了当今细胞分类器的复杂，笨重的激光和照相型探测器设备。取而代之的是，我们将使用廉价的半导体光源，例如LED，二极管激光器或VCELS，以及半导体的照相电机，例如光电二极管，二极管阵列和CCD。实际上，使用滤光到小像素簇上，单个CCD可以分析整个发射光谱。 在第一阶段，我们将在竞争的微音和微荧光（阀）方法中进行评估和选择。我们还将构建一个台式原型分类器，并演示3个平行II排序的阶段。在阶段”，我们将建立一个多阶段原型，用于由C.J. Peters博士在UTMB SL-4实验室中进行测试，这为我们提供了一个独特的机会，以获得适用于CDC A级病原体在内的生物防御应用程序的准确的生物安全测试系统。 商业机会：我们的密封，便携式和超高的吞吐量分散器将在生物防御中以及用于干细胞疗法，基因组学，蛋白质组学和组合化学应用的细胞（或珠子或颗粒）的HTP分类中广泛使用。