Automated, portable, concurrent, WMD detection system

自动化、便携式、并发大规模杀伤性武器检测系统

• 批准号: 6833762
• 负责人: Christian Schoen
• 金额: $ 49.48万
• 依托单位: CONCURRENT ANALYTICAL, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833762
• 关键词: Bacillus anthracis; Clostridium botulinum; Raman spectrometry; Yersinia pestis; air sampling /monitoring; bioengineering /biomedical engineering; biohazard detection; biomarker; biomedical automation; biomedical equipment development; bioterrorism /chemical warfare; environmental contamination; immunologic assay /test; method development; miniature biomedical equipment; molecular probes; monitoring device; nanotechnology; portable biomedical equipment; smallpox virus; time resolved data; water sampling /testing; water supply

DESCRIPTION (provided by applicant): The overall goal of this project is the development of a fully automated, wireless ethernet capable, near realtime, field deployable immunoassay-based analysis system for the concurrent, ultra low-level detection of biowarfare agents in air or water by using innovations in surface enhanced Raman scattering (SERS) and related supporting materials and hardware. As a proving ground for this concept, this Phase I effort has three major objectives. The first objective is to develop the requisite Raman active labels and sample handling protocols for the simultaneous, low-level detection (approximately 100 biolites) of simulants for Bacillus anthracis (anthrax), Clostridium botulinum (botulism), Yersinia pestis (plague), and Variola major (smallpox). The second objective is to devise a facile approach for sample concentration that will realize equilibration time's prior to readout of less than one minute. The third objective is to reduce the size and power requirements of our current NanoRamanTM I instrument to that of a 1980's cell phone. Thereby, the combined system of Extrinsic Raman Labels (ERLTM) along with the NanoRamanTM I instrument could function as a highly sensitive detection and labeling method that can be applied in a high throughput format by reading multiple biomarkers concurrently at a single biochip detector (sensor) address, and could be utilized for many portable applications. The successful completion of these three objectives will provide the framework upon which in Phase II, a detection system could be made that can detect all of the pathogens listed in Categories A, B and C that are amenable to immunoassay-based analysis, concurrently, with detection levels approaching single biolite and response times of a few minutes, in a compact, low power, portable device.

描述（由申请人提供）：该项目的总体目标是开发完全自动化的，无线以太网，近实时，现场可部署的可部署免疫测定分析系统，用于通过使用表面增强的Raman散射（SERS）和相关材料和相关材料和相关材料和相关材料和相关的材料和相关的材料和水中的创新来同时进行空气或水中生物层面的超级低级检测。作为此概念的一个证明基础，I阶段的工作具有三个主要目标。第一个目的是为同时的，低水平检测（约100个生物产物）的模拟剂开发必要的拉曼主动标签和样品处理协议，用于嗜酸芽孢杆菌（炭疽芽孢杆菌），肉毒梭菌（肉毒杆菌）（肉毒杆菌），pestis（Plague）（plague）和doviola（doviola）。第二个目标是设计一种简单的样品浓度方法，该方法将在读数不到一分钟之前实现平衡时间。第三个目标是将当前纳米剂I仪器的尺寸和功率要求降低到1980年的手机。因此，外在拉曼标签（ERLTM）的组合系统以及纳米剂I仪器可以用作高度敏感的检测和标记方法，可以通过在单个生物芯片检测器（传感器）地址同时读取多个生物标记物，以高吞吐量的格式应用，并且可以用于许多便携式应用程序。这三个目标的成功完成将提供一个框架，在第二阶段，可以制定一个检测系统，可以检测A，B和C类中列出的所有病原体，这些病原体与基于免疫测定的分析相同，同时同时基于免疫测定的分析，检测水平接近单个生物石水平，并且接近单个生物石的响应时间和几分钟的响应时间，在CARPACT，低功率，便宜的设备。