ROOT RESORPTION DUE TO ORTHODONTIC MOVEMENT

正畸移动导致的牙根吸收

• 批准号: 6731308
• 负责人: Margarita Zeichner-David
• 金额: $ 16.24万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731308
• 关键词: biological models; disease /disorder etiology; gene expression; laboratory rat; microarray technology; model design /development; molecular biology; molecular pathology; orthodontics; pathologic bone resorption; pathologic process; physiologic bone resorption; polymerase chain reaction; therapy adverse effect; tooth; tooth resorption; tooth root

DESCRIPTION (provided by applicant): Root resorption, either physiological or pathological, results in the destruction of mineralized and non-mineralized tissues of the dental roots. Pathological root resorption, internal or external, can result from the presence of cysts, tumors, trauma, infections, periodontal disease or orthodontic treatment. This process could advance in time destroying the roots and resulting in tooth loss. Apical root resorption is one of the most common problems that orthodontists face during their practice. This process could compromise the benefits of otherwise a successful orthodontic treatment and could result in severe medico-legal ramifications. Although many clinical and histological studies have been carried out in order to elucidate the etiology and pathogenesis of external root resorption, the molecular events leading to root resorption induced by orthodontic treatment are still unknown. Currently, the only available diagnosis for root resorption is by X-rays and by the time the X-ray might detect the resorption, the process is very difficult, if not impossible, to stop. Our laboratory has established a reproducible rat animal model for tooth movement resulting in tooth resorption. In the present R21 application, we request funds to create an animal model for tooth movement without root resorption, that will allow us to differentiate between genes expressed to maintain the balance between bone resorption/apposition required to move teeth, and those expressed as consequence of disruption of this balance resulting in pathological root resorption. This will be accomplished by determining the genes expressed as result of the application of orthodontic forces resulting in either tooth movement or root resorption using DNA microarrays. We will then confirm the data obtained above using RT-PCR and we will elucidate the spatial and temporal expression of candidate genes for root resorption. A better comprehension of the molecular events associated with this pathological process in the rat animal model might provide us with the starting point for future studies in humans to facilitate the treatment, prevention and possible early diagnosis of patients at risk of developing root resorption due to orthodontic treatment.

描述（由申请人提供）：生理或病理的根吸收导致牙根矿化和非矿化组织破坏。内部或外部的病理根系吸收可能是由于囊肿，肿瘤，创伤，感染，牙周疾病或正畸治疗的存在而引起的。这个过程可能会及时摧毁根部并导致牙齿脱落。根尖根吸收是正畸医生在练习过程中面临的最常见问题之一。这个过程可能会损害成功的正畸治疗的好处，并可能导致严重的医疗法律影响。尽管已经进行了许多临床和组织学研究，以阐明外部根部吸收的病因学和发病机理，但仍未知导致牙根吸收的分子事件尚不清楚。当前，唯一可用的根吸收诊断是X射线，到X射线可能检测到吸收时，该过程非常困难，即使不是不可能的停止。 我们的实验室已经建立了一种可再现的大鼠动物模型，用于牙齿运动，从而导致牙齿吸收。在当前的R21应用中，我们要求资金为无根吸收的动物模型创建一种动物模型，这将使​​我们能够区分表达的基因，以维持移动牙齿所需的骨吸收/份量之间的平衡，而那些由于这种平衡而表达的基因导致病理根吸收。这将通过确定由于使用DNA微阵列的正畸作用而表达的基因来实现。然后，我们将使用RT-PCR确认上述数据，并将阐明候选基因的空间和时间表达进行根吸收。 在大鼠动物模型中，更好地理解与该病理过程相关的分子事件可能为我们提供了人类未来研究的起点，以促进治疗，预防和可能早期诊断有正畸治疗引起的根部吸收的患者。